| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DUX2 |
| Uniprot No | O75505 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-80aa |
| 氨基酸序列 | MALPKPSDGTLPAEVQGRGQRRRLVWTPSQSKALQACFERNPYPGITTRERLAQAIGIPEPRVQILFQNERSCQLRQHWH |
| 分子量 | 8.8 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于重组人DUX2蛋白的代表性文献(虚构示例，仅供参考格式)：
1. **文献名称**: *Functional Analysis of Recombinant Human DUX2 Protein in Embryonic Stem Cell Regulation*
**作者**: Smith A, et al.
**摘要**: 通过体外表达重组人DUX2蛋白，揭示其通过结合特定基因组区域调控胚胎干细胞多能性相关基因(如OCT4)的转录，证明DUX2在早期胚胎发育中的作用。
2. **文献名称**: *DUX2-Mediated Chromatin Remodeling in Cancer Pathogenesis*
**作者**: Chen L, et al.
**摘要**: 研究重组DUX2蛋白对肿瘤细胞染色质结构的调控，发现其通过招募组蛋白修饰酶(如HDACs)促进致瘤基因沉默，提示DUX2在癌症表观遗传失调中的潜在机制。
3. **文献名称**: *Production and Characterization of Recombinant Human DUX2 for Structural Studies*
**作者**: Tanaka K, et al.
**摘要**: 报道一种高效大肠杆菌表达系统生产重组DUX2蛋白，结合X射线晶体学解析其DNA结合结构域的三维结构，为靶向DUX2的药物设计提供基础。
4. **文献名称**: *DUX2 Interaction with Non-Coding RNAs in Myogenic Differentiation*
**作者**: Park S, et al.
**摘要**: 利用重组DUX2蛋白筛选相互作用RNA分子，鉴定出多个调控肌细胞分化的长链非编码RNA，揭示DUX2通过RNA依赖途径参与肌肉发育的新功能。
注：以上文献信息为模拟示例，实际研究中请通过PubMed、Web of Science等平台以"recombinant DUX2"或"human DUX2 protein"为关键词检索真实文献。
**Background of Recombinant Human DUX2 Protein**
The human *DUX2* gene, part of the DUX (Double homeobox) family, encodes a transcription factor characterized by two homeodomains that bind DNA to regulate gene expression. DUX2 is primarily associated with early embryonic development, where it plays a role in zygotic genome activation and maintaining pluripotency in blastomeres. In somatic tissues, its expression is typically silenced due to epigenetic regulation, but aberrant reactivation occurs in certain cancers, notably subtypes of sarcoma and leukemia, where it may drive oncogenic programs by activating stem cell-related genes.
Recombinant human DUX2 protein is produced via genetic engineering for functional studies. Its truncated or full-length forms are expressed in bacterial or eukaryotic systems, often fused with tags for purification and detection. Researchers leverage this protein to investigate its DNA-binding properties, transcriptional targets, and role in cellular reprogramming or tumorigenesis. Challenges include its low stability and transient expression in vivo, complicating mechanistic studies. Nevertheless, DUX2 remains a focal point for understanding developmental biology and cancer, with therapeutic potential in targeting DUX2-driven malignancies. Current efforts also explore its utility in regenerative medicine, given its capacity to influence cell fate.
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