| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | EFCAB2 |
| Uniprot No | Q5VUJ9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-269aa |
| 氨基酸序列 | MLGPGQVRLR PRVWRDKAGG RVADGASGLP PARGSWRETG TGRALGASSP PRPAQGSSSP GIQSGPSSRP GSPRGAEQAG TPRPRLSLGI SQATGSAARW RTRRTGKGLG YNSDEIRPRT LLIEHLMEGG RRDHHTMTVL WGTQEIIVAE FHKKIKEAFE VFDHESNNTV DVREIGTIIR SLGCCPTEGE LHDLIAEVEE EEPTGYIRFE KFLPVMTEIL LERKYRPIPE DVLLRAFEVL DSAKRGFLTK DELIKYMTEE DGVSLRRPG |
| 分子量 | 29.7 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人EFCAB2蛋白的参考文献示例(文献为假设性示例，建议通过数据库进一步验证)：
1. **文献名称**: "Structural insights into calcium-binding properties of human EFCAB2"
**作者**: Smith A et al.
**摘要**: 研究通过重组人EFCAB2蛋白的体外表达和纯化，揭示了其钙离子结合结构域的三维结构，并探讨其与细胞内钙信号传导的潜在关联。
2. **文献名称**: "EFCAB2 regulates ciliary motility through interaction with microtubule complexes"
**作者**: Zhang L et al.
**摘要**: 利用重组EFCAB2蛋白进行互作实验，发现其通过结合微管相关蛋白调控纤毛运动功能，为纤毛疾病机制提供了新视角。
3. **文献名称**: "Recombinant EFCAB2 as a biomarker in cancer progression"
**作者**: Patel R et al.
**摘要**: 该研究通过重组EFCAB2蛋白验证其在多种癌细胞系中的异常表达，表明其可能通过调控细胞迁移参与肿瘤转移。
4. **文献名称**: "Functional analysis of EFCAB2 in spermatogenesis using recombinant protein models"
**作者**: Johnson M & Lee K
**摘要**: 研究利用重组EFCAB2蛋白构建体外模型，揭示了其在精子鞭毛形成中的作用及对男性生殖功能的影响。
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**注**：以上文献为模拟概括，实际研究需检索PubMed、Google Scholar等平台(关键词：**recombinant EFCAB2** / **EFCAB2 function**)。部分真实研究可能聚焦于EFCAB2与BBSome(Bardet-Biedl综合征蛋白复合体)的关联或离子通道调控。
Recombinant human EFCAB2 (EF-hand and coiled-coil domain-containing protein 2) is a calcium-binding protein implicated in cellular signaling and regulatory processes. Belonging to the EF-hand protein superfamily, EFCAB2 contains characteristic EF-hand motifs that enable calcium ion coordination, a feature critical for its interaction with downstream molecular targets. Though its precise biological functions remain under investigation, emerging studies suggest roles in modulating calcium-dependent pathways, cellular differentiation, and organelle dynamics. EFCAB2 is ubiquitously expressed across tissues, with notable presence in the brain, heart, and reproductive systems, hinting at tissue-specific regulatory functions.
Research highlights its potential involvement in cilia-associated signaling and membrane trafficking, possibly through interactions with ciliary proteins like polycystins. Dysregulation of EFCAB2 has been tentatively linked to pathologies including ciliopathies, cardiovascular disorders, and infertility. The recombinant form, typically produced in prokaryotic (e.g., *E. coli*) or eukaryotic systems, retains calcium-binding activity and serves as a key tool for structural studies, antibody development, and mechanistically exploring its interaction networks. Current investigations focus on resolving its 3D structure, calcium-sensing mechanisms, and therapeutic potential in calcium-mediated disease models. Its conserved domains across vertebrates also make it an evolutionary model for studying calcium signaling adaptations.
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