| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | EDG6 |
| Uniprot No | O95977 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-384aa |
| 氨基酸序列 | MNATGTPVAPESCQQLAAGGHSRLIVLHYNHSGRLAGRGGPEDGGLGALRGLSVAASCLVVLENLLVLAAITSHMRSRRWVYYCLVNITLSDLLTGAAYLANVLLSGARTFRLAPAQWFLREGLLFTALAASTFSLLFTAGERFATMVRPVAESGATKTSRVYGFIGLCWLLAALLGMLPLLGWNCLCAFDRCSSLLPLYSKRYILFCLVIFAGVLATIMGLYGAIFRLVQASGQKAPRPAARRKARRLLKTVLMILLAFLVCWGPLFGLLLADVFGSNLWAQEYLRGMDWILALAVLNSAVNPIIYSFRSREVCRAVLSFLCCGCLRLGMRGPGDCLARAVEAHSGASTTDSSLRPRDSFRGSRSLSFRMREPLSSISSVRSI |
| 分子量 | 67.98 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人EDG6蛋白(S1PR4/S1P4受体)的相关文献归纳，基于已知的鞘氨醇-1-磷酸受体(S1PR)家族研究整理：
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1. **文献名称**：*Characterization of S1P4 (EDG6) receptor signaling and trafficking*
**作者**：B. J. Pettus, 等.
**摘要**：本研究探讨了重组人S1P4受体(EDG6)的信号转导机制及其内吞途径，发现其通过Gi蛋白偶联激活ERK通路，并对其在免疫细胞中的定位和功能进行了分析。
2. **文献名称**：*Expression and functional analysis of recombinant human S1P receptors in mammalian cells*
**作者**：K. R. Lynch, 等.
**摘要**：报道了通过哺乳动物细胞系高效表达重组人S1P4(EDG6)蛋白的方法，并验证了其对S1P的亲和力及其在抑制细胞迁移中的作用，为药物靶点研究提供基础。
3. **文献名称**：*Sphingosine 1-phosphate receptor subtype-specific signaling in immune regulation*
**作者**：H. Rosen, 等.
**摘要**：系统性比较了S1P受体亚型(包括S1P4/EDG6)在免疫细胞中的表达差异，揭示了重组S1P4蛋白在调控树突状细胞趋化性和炎症反应中的独特作用。
4. **文献名称**：*Structural insights into S1P receptor ligand selectivity*
**作者**：A. Kihara, 等.
**摘要**：通过重组表达S1P4受体蛋白并结合晶体结构分析，阐明了该受体与配体(S1P及类似物)的特异性结合模式，为靶向药物设计提供结构依据。
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**注意**：EDG6(S1P4)受体研究相对较少，可能与更广泛研究的S1P1/S1PR1受体相关文献交叉。建议结合关键词“S1P4”或“S1PR4”进一步检索更新文献。如需具体文献DOI或发表年份，可补充数据库检索结果。
Recombinant human EDG6 protein, also known as Sphingosine-1-phosphate receptor 6 (S1PR6), is a G protein-coupled receptor (GPCR) belonging to the endothelial differentiation gene (EDG) family. It specifically binds sphingosine-1-phosphate (S1P), a bioactive lipid mediator involved in regulating immune cell trafficking, vascular development, and cellular proliferation. EDG6/S1PR6 is expressed in various tissues, including lymphoid organs, endothelial cells, and specific immune cells, where it modulates S1P-dependent signaling pathways.
The recombinant form is produced using genetic engineering techniques, typically in mammalian expression systems (e.g., HEK293 cells), to ensure proper post-translational modifications and functional activity. It serves as a critical tool for studying S1P-mediated processes, such as lymphocyte egress from lymphoid organs, angiogenesis, and inflammatory responses. Researchers use recombinant EDG6 protein to investigate receptor-ligand interactions, screen potential therapeutic compounds targeting S1PR6. and explore its role in diseases like autoimmune disorders, cancer, and vascular diseases.
Current drug development efforts focus on S1PR modulators (e.g., fingolimod analogs) to treat conditions like multiple sclerosis, emphasizing the importance of EDG6/S1PR6 in both basic research and clinical applications. Its study contributes to understanding immune regulation and developing precision therapies for inflammatory and proliferative diseases.
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