| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | EDG1 |
| Uniprot No | P21453 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-382aa |
| 氨基酸序列 | MGPTSVPLVKAHRSSVSDYVNYDIIVRHYNYTGKLNISADKENSIKLTSVVFILICCFIILENIFVLLTIWKTKKFHRPMYYFIGNLALSDLLAGVAYTANLLLSGATTYKLTPAQWFLREGSMFVALSASVFSLLAIAIERYITMLKMKLHNGSNNFRLFLLISACWVISLILGGLPIMGWNCISALSSCSTVLPLYHKHYILFCTTVFTLLLLSIVILYCRIYSLVRTRSRRLTFRKNISKASRSSEKSLALLKTVIIVLSVFIACWAPLFILLLLDVGCKVKTCDILFRAEYFLVLAVLNSGTNPIIYTLTNKEMRRAFIRIMSCCKCPSGDSAGKFKRPIIAGMEFSRSKSDNSSHPQKDEGDNPETIMSSGNVNSSS |
| 分子量 | 69.2 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人EDG1(S1PR1)蛋白的3篇代表性文献示例(注：文献为示例，实际引用需核实)：
1. **《Characterization of Recombinant Human S1P Receptor EDG1 Signaling》**
- 作者：Lee, M.J. et al.
- 摘要：通过昆虫细胞系统表达重组人EDG1蛋白，验证其与S1P(鞘氨醇-1-磷酸)的特异性结合，并阐明其在调控细胞迁移和血管生成中的作用。
2. **《Structural Basis of S1P Recognition by EDG1》**
- 作者：Im, D.S. et al.
- 摘要：利用X射线晶体学解析重组EDG1蛋白的3D结构，揭示其与S1P结合的位点，为靶向EDG1的药物设计提供分子基础。
3. **《EDG1/S1P1-mediated Signal Transduction in Immune Cell Regulation》**
- 作者：Spiegel, S. et al.
- 摘要：研究重组EDG1在T细胞中的功能，证明其通过S1P信号通路参与淋巴细胞迁移和自身免疫疾病的病理机制。
4. **《Expression and Functional Analysis of EDG1 in Cancer Models》**
- 作者：Hla, T. et al.
- 摘要：构建重组EDG1过表达细胞模型，揭示其在肿瘤血管生成和转移中的作用，为癌症治疗提供潜在靶点。
(注：EDG1现多被称为S1PR1.建议以最新基因命名为关键词检索具体文献。)
Recombinant human EDG1 protein, also known as Sphingosine-1-phosphate receptor 1 (S1PR1), is a engineered protein produced through genetic recombination technology. EDG1 belongs to the endothelial differentiation gene (EDG) family of G protein-coupled receptors, which specifically binds sphingosine-1-phosphate (S1P), a bioactive lipid mediator. It plays critical roles in regulating immune cell trafficking, vascular development, and cellular responses like proliferation, migration, and survival. Structurally, it contains seven transmembrane domains typical of GPCRs. Research on recombinant EDG1 has advanced understanding of its involvement in pathological conditions including cancer metastasis, inflammatory diseases, and autoimmune disorders like multiple sclerosis. The development of recombinant EDG1 protein facilitates functional studies, drug screening, and therapeutic targeting, particularly as S1PR1 modulators (e.g., fingolimod) have been approved for clinical use. Its recombinant form allows standardized in vitro experiments to explore signaling mechanisms or evaluate potential inhibitors. Current studies also focus on its dual role in promoting or suppressing diseases depending on cellular context, highlighting its complex regulatory networks. This protein remains a key subject in immunology and precision medicine research.
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