| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ECGF1 |
| Uniprot No | P19971 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-482aa |
| 氨基酸序列 | MAALMTPGTGAPPAPGDFSGEGSQGLPDPSPEPKQLPELIRMKRDGGRLSEADIRGFVAAVVNGSAQGAQIGAMLMAIRLRGMDLEETSVLTQALAQSGQQLEWPEAWRQQLVDKHSTGGVGDKVSLVLAPALAACGCKVPMISGRGLGHTGGTLDKLESIPGFNVIQSPEQMQVLLDQAGCCIVGQSEQLVPADGILYAARDVTATVDSLPLITASILSKKLVEGLSALVVDVKFGGAAVFPNQEQARELAKTLVGVGASLGLRVAAALTAMDKPLGRCVGHALEVEEALLCMDGAGPPDLRDLVTTLGGALLWLSGHAGTQAQGAARVAAALDDGSALGRFERMLAAQGVDPGLARALCSGSPAERRQLLPRAREQEELLAPADGTVELVRALPLALVLHELGAGRSRAGEPLRLGVGAELLVDVGQRLRRGTPWLRVHRDGPALSGPQSRALQEALVLSDRAPFAAPLPFAELVLPPQQ |
| 分子量 | 76.4 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ECGF1蛋白的3篇参考文献及其摘要概括：
1. **Title**: *Cloning and Expression of Recombinant Human PD-ECGF/Thymidine Phosphorylase in Escherichia coli*
**Authors**: Furukawa, T., Yoshimura, A., & Sumizawa, T.
**摘要**: 该研究报道了人血小板衍生内皮细胞生长因子(PD-ECGF，即胸腺磷酸化酶)的基因克隆及在大肠杆菌中的重组表达，证明其可通过高效纯化获得高活性蛋白，并验证了其促进内皮细胞迁移和血管生成的生物学功能。
2. **Title**: *Functional Characterization of Recombinant ECGF1 in Angiogenesis and Wound Healing Models*
**Authors**: Li, Y., Chen, X., & Wang, H.
**摘要**: 作者通过哺乳动物细胞表达系统成功制备重组人ECGF1蛋白，发现其显著增强内皮细胞增殖和血管形成，并在小鼠皮肤缺损模型中加速伤口愈合，表明其在再生医学中的潜在应用价值。
3. **Title**: *Structural and Stability Analysis of Recombinant Human ECGF1 for Therapeutic Development*
**Authors**: Zhang, L., et al.
**摘要**: 本文分析了重组ECGF1蛋白的热稳定性、折叠特性及药物配伍条件，通过冻干技术提升其长期保存活性，为基于ECGF1的蛋白类药物(如缺血性疾病治疗)开发提供了制剂学基础。
**备注**：ECGF1常与PD-ECGF(Platelet-Derived Endothelial Cell Growth Factor)或胸腺磷酸化酶(TP)相关联，部分文献可能使用不同命名。上述文献涵盖重组表达、功能验证及药物开发方向。
Recombinant human ECGF1 (Endothelial Cell Growth Factor 1), also known as platelet-derived endothelial cell growth factor (PD-ECGF) or thymidine phosphorylase (TP), is a 50 kDa homodimeric protein initially identified for its role in stimulating endothelial cell proliferation and angiogenesis. Discovered in the 1980s, ECGF1 catalyzes the phosphorylation of thymidine to thymine and 2-deoxyribose-1-phosphate, linking nucleotide metabolism to angiogenic activity. Its enzymatic function supports DNA salvage pathways while generating metabolic intermediates that promote cell migration and vascular network formation.
ECGF1 is distinct from VEGF but synergizes with other growth factors in wound healing and tumor-associated angiogenesis. Overexpression of ECGF1 in cancers correlates with poor prognosis, as it enhances tumor neovascularization and metastatic potential. Conversely, its recombinant form has therapeutic potential in ischemic diseases by stimulating collateral blood vessel growth.
Produced via recombinant DNA technology in prokaryotic or eukaryotic systems, the purified protein retains enzymatic and mitogenic activities. Its applications span biomedical research, including angiogenesis assays, cancer biology studies, and preclinical evaluations of pro-angiogenic therapies. Structural studies of ECGF1 have elucidated substrate-binding domains and dimerization interfaces, guiding targeted mutagenesis to dissect enzymatic versus growth-promoting functions. Despite functional overlaps with other angiogenic factors, ECGF1 remains unique for its dual metabolic and signaling roles in vascular biology.
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