| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | EBF4 |
| Uniprot No | Q9BQW3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-88aa |
| 氨基酸序列 | MPSSPPLAAASSMSLPAAAPTTSVFSFSPVNMISAVKQRSAFAPVLRPPSSPPQACPRAHGEGLPDQSFEDSDKFHSPARGLQGLAYS |
| 分子量 | 35.5 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人EBF4蛋白的示例参考文献(注：以下内容为假设性文献示例，仅供格式参考)：
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1. **文献名称**: *Functional Characterization of Recombinant Human EBF4 in B-Cell Differentiation*
**作者**: Smith J, Brown A, Lee C
**摘要**: 本研究利用大肠杆菌表达系统制备了重组人EBF4蛋白，并证明其通过激活特定B细胞基因(如CD79A)调控早期B细胞分化的功能。
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2. **文献名称**: *Structural Insights into EBF4 DNA-Binding Domain via X-ray Crystallography*
**作者**: Zhang Y, Wang H, Kumar S
**摘要**: 通过重组表达人EBF4蛋白的DNA结合结构域，结合晶体学分析揭示其与靶DNA序列的相互作用机制，为EBF家族转录因子的功能研究提供结构基础。
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3. **文献名称**: *EBF4 Overexpression in Neural Stem Cells Promotes Neuronal Maturation*
**作者**: Garcia R, Chen L, Patel D
**摘要**: 使用重组EBF4蛋白在小鼠神经干细胞中过表达，发现其通过调节NeuroD1等关键基因，显著增强神经元成熟和突触形成能力。
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4. **文献名称**: *Recombinant EBF4 Interaction with E2A Proteins in Transcriptional Regulation*
**作者**: Johnson M, Kim T, Adams R
**摘要**: 通过体外结合实验证实重组人EBF4蛋白与E2A转录因子形成复合物，协同激活免疫相关基因的表达，提示其在调控淋巴系统发育中的协同作用。
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**备注**：以上文献为示例，实际引用需根据真实研究调整。如需查找真实文献，建议在PubMed或Web of Science中搜索关键词“Recombinant EBF4”或“Human EBF4 protein function”。
Recombinant human EBF4 (Early B-Cell Factor 4) is a protein encoded by the *EBF4* gene, belonging to the EBF family of transcription factors. These proteins are characterized by a conserved DNA-binding domain that facilitates interactions with specific promoter regions, regulating gene expression. EBF4 is notably involved in cellular differentiation, tissue development, and maintaining cellular identity, particularly in neural and mesenchymal tissues. Dysregulation of EBF4 has been linked to various diseases, including cancers and neurodevelopmental disorders, underscoring its role in critical biological processes.
Produced via recombinant DNA technology, the EBF4 protein is typically expressed in systems like *E. coli* or mammalian cell cultures, followed by purification steps to ensure high specificity and activity. The recombinant form allows researchers to study its molecular functions, such as DNA-binding kinetics, transcriptional activation/repression, and interactions with co-regulators. Its applications span basic research—exploring mechanisms of gene regulation—to potential therapeutic development, such as targeting pathways in EBF4-associated diseases. As a tool, recombinant EBF4 aids in dissecting its role in developmental biology and disease pathology, offering insights into novel biomarkers or therapeutic strategies. Ongoing studies continue to unravel its context-dependent functions across different tissues and disease models.
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