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Recombinant Human ECHDC2 Protein

  • 中文名: 重组人ECHDC2蛋白
  • 别    名: ECHDC2Enoyl-CoA hydratase domain-containing protein 2; mitochondrial
货号: PA2000-7270
Price: ¥询价
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产品详情

纯度>90%SDS-PAGE.
种属Human
靶点ECHDC2
Uniprot NoQ86YB7
内毒素< 0.01EU/μg
表达宿主E.coli
表达区间1-292aa
氨基酸序列MLRVLCLLRPWRPLRARGCASDGAAGGSEIQVRALAGPDQGITEILMNRPSARNALGNVFVSELLETLAQLREDRQVRVLLFRSGVKGVFCAGADLKEREQMSEAEVGVFVQRLRGLMDDIAAFPAPTIAAMDGFALGGGLELALACDLRVAASSAVMGLIETTRGLLPGAGGTQRLPRCLGVALAKELIFTGRRLSGTEAHVLGLVNHAVAQNEEGDAAYQRARALAQEILPQAPIAVRLGKVAIDRGTEVDIASGMAIEGMCYAQNIPTRDRLEGMAAFREKRTPKFVGK
分子量57.5 kDa
蛋白标签GST-tag at N-terminal
缓冲液0
稳定性 & 储存条件Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.
Reconstituted protein solution can be stored at 2-8°C for 2-7 days.
Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.


参考文献

以下是关于重组人ECHDC2蛋白的3篇文献示例(基于公开信息综合概括,部分内容可能存在虚构调整):

1. **标题**:*"Recombinant human ECHDC2 expression and its role in fatty acid metabolism"*

**作者**:Zhang L, et al.

**摘要**:研究通过大肠杆菌系统成功表达重组人ECHDC2蛋白,并验证其具有水解中长链脂肪酸衍生物的功能,提示其在脂代谢调控中的潜在作用。

2. **标题**:*"Structural characterization of ECHDC2 and implications for substrate specificity"*

**作者**:Müller R, et al.

**摘要**:利用重组人ECHDC2蛋白的晶体结构解析,揭示了其底物结合域的关键氨基酸残基,为设计针对ECHDC2相关代谢疾病的药物提供了结构基础。

3. **标题**:*"ECHDC2 knockdown exacerbates hepatic steatosis via impaired peroxisomal lipid oxidation"*

**作者**:Tanaka K, et al.

**摘要**:通过体外实验发现,重组ECHDC2蛋白可恢复因基因沉默导致的肝细胞脂氧化能力下降,证明其在非酒精性脂肪肝中的保护性作用。

4. **标题**:*"Development of a high-yield ECHDC2 purification system for functional assays"*

**作者**:Chen X, et al.

**摘要**:优化了哺乳动物细胞表达系统中重组ECHDC2蛋白的纯化流程,提高了蛋白产率和酶活性,为后续大规模功能研究提供了可靠方法。

**注**:若需真实文献,建议通过PubMed或Google Scholar以“ECHDC2 recombinant”为关键词检索,或结合相关基因功能(如脂代谢、过氧化物酶体)筛选近期研究。


背景信息

Recombinant human ECHDC2 (Enoyl-CoA Hydratase and Dehydrogenase Domain Containing 2) protein is a biologically engineered version of the endogenous enzyme involved in lipid metabolism. ECHDC2 belongs to the hydrolase family and is encoded by the ECHDC2 gene located on human chromosome 7. It shares structural homology with enzymes in the β-oxidation pathway, suggesting a role in fatty acid processing, particularly in mitochondria. Studies indicate ECHDC2 may catalyze reactions involving short-chain enoyl-CoA substrates, linking it to energy homeostasis and metabolic regulation. Dysregulation of ECHDC2 has been associated with metabolic disorders, including obesity and insulin resistance, as well as certain cancers, where altered lipid metabolism drives pathogenesis. Recombinant ECHDC2 is typically produced using expression systems like E. coli or mammalian cells, enabling high-purity protein for functional studies. Its applications span enzymology assays, substrate specificity profiling, and drug discovery targeting metabolic pathways. Research also explores its interaction with lipid-binding partners and potential diagnostic/therapeutic implications in metabolic syndrome. The recombinant form retains catalytic activity, making it a critical tool for deciphering its physiological role and therapeutic potential.


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