| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RRM2B |
| Uniprot No | Q7LG56 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-351aa |
| 氨基酸序列 | MGDPERPEAAGLDQDERSSSDTNESEIKSNEEPLLRKSSRRFVIFPIQYPDIWKMYKQAQASFWTAEEVDLSKDLPHWNKLKADEKYFISHILAFFAASDGIVNENLVERFSQEVQVPEARCFYGFQILIENVHSEMYSLLIDTYIRDPKKREFLFNAIETMPYVKKKADWALRWIADRKSTFGERVVAFAAVEGVFFSGSFAAIFWLKKRGLMPGLTFSNELISRDEGLHCDFACLMFQYLVNKPSEERVREIIVDAVKIEQEFLTEALPVGLIGMNCILMKQYIEFVADRLLVELGFSKVFQAENPFDFMENISLEGKTNFFEKRVSEYQRFAVMAETTDNVFTLDADF |
| 预测分子量 | 67.7kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RRM2B重组蛋白的3篇参考文献示例(注：以下为虚拟文献，实际引用需查询具体数据库)：
1. **文献名称**：*"Expression and Functional Characterization of Recombinant Human RRM2B Protein in Mitochondrial DNA Maintenance"*
**作者**：Chen L., et al.
**摘要**：本研究成功在大肠杆菌中表达并纯化了重组人源RRM2B蛋白，证实其具有核糖核苷酸还原酶活性，并在线粒体DNA损伤修复中起关键作用。
2. **文献名称**：*"Structural Insights into RRM2B Recombinant Protein and Its Role in Oxidative Stress Response"*
**作者**：Kim S., Park J.
**摘要**：通过X射线晶体学解析了RRM2B重组蛋白的三维结构，揭示了其与氧化应激条件下核苷酸代谢相关的功能机制，为相关疾病治疗提供理论依据。
3. **文献名称**：*"Development of a Stable RRM2B Recombinant Protein Delivery System for Cancer Therapy"*
**作者**：Garcia R., et al.
**摘要**：开发了一种基于腺病毒载体的RRM2B重组蛋白递送系统，证明其在抑制肿瘤细胞增殖和增强化疗敏感性中的潜在应用价值。
The ribonucleotide reductase regulatory subunit B2 (RRM2B), also known as p53-inducible ribonucleotide reductase (p53R2), is a critical enzyme involved in DNA synthesis and repair. It functions as a regulatory component of the ribonucleotide reductase (RNR) complex, which catalyzes the conversion of ribonucleotides to deoxyribonucleotides (dNTPs), essential precursors for DNA replication and repair. Unlike its homolog RRM2. which is cell cycle-regulated, RRM2B is constitutively expressed and plays a vital role in maintaining mitochondrial DNA (mtDNA) integrity by supplying dNTPs to mitochondria. This function is particularly important in non-dividing cells and under stress conditions, such as oxidative damage or p53 activation during genotoxic stress.
RRM2B gained attention due to its direct regulation by the tumor suppressor protein p53. linking it to p53-mediated DNA repair and apoptosis. Mutations in the RRM2B gene are associated with severe mitochondrial disorders, including encephalomyopathy, mtDNA depletion syndromes, and progressive external ophthalmoplegia. These conditions highlight its indispensable role in mtDNA maintenance.
Recombinant RRM2B protein is produced using expression systems like E. coli or mammalian cells, enabling studies on its enzymatic activity, structural properties, and interactions with other RNR subunits. Its recombinant form is instrumental in investigating mitochondrial dNTP pool regulation, DNA damage responses, and therapeutic strategies for mitochondrial diseases or cancers. Researchers also explore RRM2B's potential as a biomarker or target for chemotherapy sensitization, given its dual role in nuclear and mitochondrial genome stability.
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