Recombinant mouse CSF1R protein

中文名： 小鼠巨噬细胞集落刺激因子1受体(CSF1R)重组蛋白
别名： CSF1R；FMS；Macrophage colony-stimulating factor 1 receptor

货号: PA1000-7582

Price： ￥询价

20μg 50μg 100μg ＞100μg

数量：

大包装询价

产品详情

纯度	>90%SDS-PAGE.
种属	mouse
靶点	CSF1R
Uniprot No	P09581
内毒素	< 0.01EU/μg
表达宿主	E.coli
表达区间	20-511aa
氨基酸序列	APVIEPSGPELVVEPGETVTLRCVSNGSVEWDGPISPYWTLDPESPGSTL TTRNATFKNTGTYRCTELEDPMAGSTTIHLYVKDPAHSWNLLAQEVTVVE GQEAVLPCLITDPALKDSVSLMREGGRQVLRKTVYFFSPWRGFIIRKAKV LDSNTYVCKTMVNGRESTSTGIWLKVNRVHPEPPQIKLEPSKLVRIRGEA AQIVCSATNAEVGFNVILKRGDTKLEIPLNSDFQDNYYKKVRALSLNAVD FQDAGIYSCVASNDVGTRTATMNFQVVESAYLNLTSEQSLLQEVSVGDSL ILTVHADAYPSIQHYNWTYLGPFFEDQRKLEFITQRAIYRYTFKLFLNRV KASEAGQYFLMAQNKAGWNNLTFELTLRYPPEVSVTWMPVNGSDVLFCDV SGYPQPSVTWMECRGHTDRCDEAQALQVWNDTHPEVLSQKPFDKVIIQSQ LPIGTLKHNMTYFCKTHNSVGNSSQYFRAVSLGQSKQLPDES
预测分子量	81 kDa
蛋白标签	His tag N-Terminus
缓冲液	PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

1. **"Structural basis of the CSF-1R/c-KIT selectivity by a series of 2.4-diaminopyrimidine derivatives" - Author: Zhang et al.**

摘要：通过X射线晶体学解析了CSF1R重组蛋白与抑制剂的复合物结构，阐明了小分子选择性靶向CSF1R的分子机制，为药物设计提供结构基础。

2. **"Expression and functional characterization of recombinant human CSF1R in insect cells" - Author: Stanley et al.**

摘要：利用杆状病毒-昆虫细胞系统表达功能性CSF1R胞外域重组蛋白，验证其与配体CSF-1的结合活性及下游信号激活能力，建立高效蛋白生产方法。

3. **"CSF1R extracellular domain mutation effects on receptor dimerization and signaling" - Author: Verstraete et al.**

摘要：通过构建CSF1R重组蛋白突变体，分析胞外域关键氨基酸对受体二聚化及激酶活性的调控作用，揭示致病性突变导致受体异常激活的机制。

4. **"Development of a CSF1R kinase assay using recombinant protein for high-throughput screening" - Author: Li et al.**

摘要：基于大肠杆菌表达的CSF1R激酶结构域重组蛋白，建立高通量抑制剂筛选平台，鉴定出多个具有纳摩尔级抑制活性的先导化合物。

背景信息

The colony-stimulating factor 1 receptor (CSF1R), also known as the macrophage colony-stimulating factor receptor (M-CSFR), is a cell-surface tyrosine kinase receptor encoded by the *CSF1R* gene. It plays a critical role in regulating the survival, proliferation, differentiation, and functional activation of mononuclear phagocytes, including monocytes, macrophages, and osteoclasts. CSF1R binds to its primary ligands, CSF1 and interleukin-34 (IL-34), triggering receptor dimerization and activation of downstream signaling pathways such as MAPK, PI3K/AKT, and JAK/STAT. These pathways are essential for maintaining immune homeostasis, tissue remodeling, and inflammatory responses.

Recombinant CSF1R proteins are engineered versions of the receptor, typically produced in mammalian or insect cell systems to ensure proper post-translational modifications, such as glycosylation. These proteins often include the extracellular ligand-binding domain and are used as research tools to study receptor-ligand interactions, signaling mechanisms, and drug screening. For instance, recombinant CSF1R is employed in binding assays to evaluate inhibitors targeting CSF1R-driven pathways, which are implicated in diseases like cancer, rheumatoid arthritis, and neurodegenerative disorders.

In therapeutic contexts, dysregulated CSF1R signaling is linked to tumor-associated macrophages (TAMs) that promote tumor progression and immunosuppression. Thus, recombinant CSF1R aids in developing monoclonal antibodies or small-molecule inhibitors (e.g., pexidartinib) to modulate CSF1R activity. Additionally, it serves as a critical reagent in preclinical models to explore CSF1R's role in diseases and validate targeted therapies. Overall, recombinant CSF1R bridges basic research and clinical applications, offering insights into immune regulation and therapeutic innovation.