| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | EMR3 |
| Uniprot No | Q9BY15 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-652aa |
| 氨基酸序列 | MQGPLLLPGLCFLLSLFGAVTQKTKTSCAKCPPNASCVNNTHCTCNHGYTSGSGQKLFTFPLETCNDINECTPPYSVYCGFNAVCYNVEGSFYCQCVPGYRLHSGNEQFSNSNENTCQDTTSSKTTEGRKELQKIVDKFESLLTNQTLWRTEGRQEISSTATTILRDVESKVLETALKDPEQKVLKIQNDSVAIETQAITDNCSEERKTFNLNVQMNSMDIRCSDIIQGDTQGPSAIAFISYSSLGNIINATFFEEMDKKDQVYLNSQVVSAAIGPKRNVSLSKSVTLTFQHVKMTPSTKKVFCVYWKSTGQGSQWSRDGCFLIHVNKSHTMCNCSHLSSFAVLMALTSQEEDPVLTVITYVGLSVSLLCLLLAALTFLLCKAIRNTSTSLHLQLSLCLFLAHLLFLVGIDRTEPKVLCSIIAGALHYLYLAAFTWMLLEGVHLFLTARNLTVVNYSSINRLMKWIMFPVGYGVPAVTVAISAASWPHLYGTADRCWLHLDQGFMWSFLGPVCAIFSANLVLFILVFWILKRKLSSLNSEVSTIQNTRMLAFKATAQLFILGCTWCLGLLQVGPAAQVMAYLFTIINSLQGFFIFLVYCLLSQQVQKQYQKWFREIVKSKSESETYTLSSKMGPDSKPSEGDVFPGQVKRKY |
| 分子量 | 72.6 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是与重组人EMR3蛋白相关的3篇参考文献示例：
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1. **文献名称**：*"Recombinant expression and functional analysis of human EMR3 in leukocyte chemotaxis"*
**作者**：Chen, L., et al. (2017)
**摘要**：研究通过哺乳动物细胞系统成功表达并纯化重组人EMR3胞外域，证实其通过结合CD97调节中性粒细胞的趋化作用，提示其在炎症反应中的潜在调控机制。
2. **文献名称**：*"Structural characterization of the EMR3 extracellular domain and its interaction with chondroitin sulfate"*
**作者**：Kumar, S., et al. (2019)
**摘要**：通过重组表达EMR3胞外区并结合质谱分析，揭示其与硫酸软骨素多糖的特异性结合，为EMR3在细胞黏附及肿瘤微环境中的作用提供结构依据。
3. **文献名称**：*"Recombinant EMR3 protein modulates macrophage polarization in vitro"*
**作者**：Zhang, Y., et al. (2020)
**摘要**：利用重组EMR3蛋白处理巨噬细胞，发现其通过ERK信号通路促进M2型极化，暗示EMR3可能在炎症性疾病治疗中具有应用潜力。
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注：上述文献信息为示例，实际引用时需根据具体研究核实准确性。建议通过PubMed或Google Scholar搜索关键词**“recombinant human EMR3”**获取最新研究。
Recombinant human EMR3 (EGF-like module-containing mucin-like hormone receptor 3) is a protein of interest in immunology and transmembrane signaling research. Belonging to the adhesion G protein-coupled receptor (GPCR) family, EMR3 is primarily expressed on immune cells, including neutrophils, macrophages, and dendritic cells. It features a characteristic structure: an extracellular N-terminal region with multiple EGF-like domains and a mucin-like stalk, a transmembrane domain, and a short intracellular C-terminus. These structural elements suggest roles in cell-cell interactions and immune regulation.
EMR3 interacts with CD97. another adhesion GPCR, modulating inflammatory responses and phagocytosis. Studies link EMR3 to diseases such as chronic obstructive pulmonary disease (COPD), rheumatoid arthritis, and cancer, where its expression correlates with immune cell activation or tumor progression. Recombinant EMR3 protein, typically produced in mammalian expression systems to ensure proper post-translational modifications, enables functional studies, ligand-binding assays, and therapeutic target exploration. Its role in immune homeostasis and disease pathology makes it a potential biomarker or therapeutic target, though mechanistic details remain under investigation. Current research focuses on elucidating its signaling pathways and tissue-specific functions.
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