| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ENAH |
| Uniprot No | Q8N8S7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-591aa |
| 氨基酸序列 | MSEQSICQAR AAVMVYDDAN KKWVPAGGST GFSRVHIYHH TGNNTFRVVG RKIQDHQVVI NCAIPKGLKY NQATQTFHQW RDARQVYGLN FGSKEDANVF ASAMMHALEV LNSQETGPTL PRQNSQLPAQ VQNGPSQEEL EIQRRQLQEQ QRQKELERER LERERMERER LERERLERER LERERLEQEQ LERERQERER QERLERQERL ERQERLERQE RLDRERQERQ ERERLERLER ERQERERQEQ LEREQLEWER ERRISSAAAP ASVETPLNSV LGDSSASEPG LQAASQPAET PSQQGIVLGP LAPPPPPPLP PGPAQASVAL PPPPGPPPPP PLPSTGPPPP PPPPPLPNQV PPPPPPPPAP PLPASGFFLA SMSEDNRPLT GLAAAIAGAK LRKVSRMEDT SFPSGGNAIG VNSASSKTDT GRGNGPLPLG GSGLMEEMSA LLARRRRIAE KGSTIETEQK EDKGEDSEPV TSKASSTSTP EPTRKPWERT NTMNGSKSPV ISRRDSPRKN QIVFDNRSYD SLHRPKSTPL SQPSANGVQT EGLDYDRLKQ DILDEMRKEL TKLKEELIDA IRQELSKSNT A |
| 分子量 | 66.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ENAH蛋白的3篇参考文献示例，涵盖表达制备、结构分析和功能研究等方向：
1. **文献名称**: *"Expression and Purification of Recombinant Human ENAH for Functional Studies"*
**作者**: Müller R, et al.
**摘要**: 研究利用昆虫细胞表达系统成功表达并纯化了重组人ENAH蛋白，验证了其与肌动蛋白结合的能力，为后续功能分析提供工具。
2. **文献名称**: *"Crystal Structure of Recombinant ENAH Reveals Actin-Binding Mechanism"*
**作者**: Tanaka K, Watanabe T
**摘要**: 通过X射线晶体学解析重组ENAH的三维结构，阐明其EVH1结构域与细胞骨架蛋白相互作用的分子机制，支持其在细胞迁移中的作用。
3. **文献名称**: *"Recombinant ENAH Promotes Breast Cancer Metastasis via Epithelial-Mesenchymal Transition"*
**作者**: Li X, et al.
**摘要**: 体外实验表明，重组ENAH蛋白通过调控上皮间质转化(EMT)增强乳腺癌细胞的迁移和侵袭能力，提示其作为潜在治疗靶点。
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**注**：上述文献为示例性内容，实际引用时请以真实文献为准。如需查找具体文献，建议在PubMed或Web of Science中使用关键词“recombinant human ENAH”或“hMena protein”进行检索。
Recombinant human ENAH (Enabled homolog) protein, a member of the Ena/VASP (Enabled/Vasodilator-Stimulated Phosphoprotein) family, plays a pivotal role in regulating actin cytoskeleton dynamics. ENAH is critical for cell motility, adhesion, and morphogenesis by promoting actin polymerization and filament bundling. It contains conserved EVH1 (Ena/VASP homology 1) and EVH2 domains, mediating interactions with signaling adaptors and actin regulators. Dysregulation of ENAH is linked to cancer metastasis, neurodevelopmental disorders, and immune cell dysfunction, highlighting its biomedical relevance.
Produced via recombinant DNA technology, human ENAH is typically expressed in Escherichia coli or mammalian systems, enabling high-purity yields for functional studies. Tags like His or GST facilitate purification and detection. Recombinant ENAH allows in vitro investigation of its biochemical properties, such as binding partners (e.g., Profilin, Mena), actin remodeling mechanisms, and phosphorylation-dependent signaling. It also serves as a tool for screening therapeutic agents targeting cytoskeleton-driven pathologies, including invasive cancers. Studies using recombinant ENAH have advanced understanding of how cytoskeletal rewiring contributes to disease progression, offering potential pathways for intervention. This protein remains a key reagent in cell biology and translational research focused on metastasis and tissue development.
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