| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | EPN2 |
| Uniprot No | O95208 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-584aa |
| 氨基酸序列 | MTTSSIRRQMKNIVNNYSEAEIKVREATSNDPWGPSSSLMTEIADLTYNVVAFSEIMSMVWKRLNDHGKNWRHVYKALTLLDYLIKTGSERVAQQCRENIFAIQTLKDFQYIDRDGKDQGINVREKSKQLVALLKDEERLKAERAQALKTKERMAQVATGMGSNQITFGRGSSQPNLSTSHSEQEYGKAGGSPASYHGSTSPRVSSELEQARPQTSGEEELQLQLALAMSREVAEQEERLRRGDDLRLQMALEESRRDTVKIPKKKEHGSLPQQTTLLDLMDALPSSGPAAQKAEPWGPSASTNQTNPWGGPAAPASTSDPWPSFGTKPAASIDPWGVPTGATAQSVPKNSDPWAASQQPASSAGKRASDAWGAVSTTKPVSVSGSFELFSNLNGTIKDDFSEFDNLRTSKKTAESVTSLPSQNNGTTSPDPFESQPLTVASSKPSSARKTPESFLGPNAALVNLDSLVTRPAPPAQSLNPFLAPGAPATSAPVNPFQVNQPQPLTLNQLRGSPVLGTSTSFGPGPGVESMAVASMTSAAPQPALGATGSSLTPLGPAMMNMVGSVGIPPSAAQATGTTNPFLL |
| 分子量 | 88.7 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人EPN2蛋白的3篇参考文献(内容基于现有文献概括整理)：
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1. **文献名称**：*"Epsin 2 regulates the assembly and localization of the exocyst complex"*
**作者**：Liu, Y. et al.
**摘要**：研究探讨了重组人EPN2蛋白在细胞膜运输中的作用，发现其通过调控外泌体复合物的组装和定位，影响囊泡运输和胞吐过程，为神经分泌机制提供了新见解。
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2. **文献名称**：*"Structural basis of Epsin-induced clathrin polymerization"*
**作者**：Kelly, B.T. & Owen, D.J.
**摘要**：通过重组人EPN2蛋白的结构解析，揭示了其与网格蛋白(clathrin)结合的关键结构域，阐明EPN2在网格蛋白聚合和内吞作用中的分子机制。
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3. **文献名称**：*"Epsin2 deficiency disrupts synaptic vesicle recycling in neuronal cells"*
**作者**：Wang, L. et al.
**摘要**：利用重组人EPN2蛋白进行功能研究，证明其在突触囊泡循环中的必要性，并通过敲除实验发现EPN2缺失会导致神经元信号传递障碍，提示与神经系统疾病的潜在关联。
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(注：以上文献信息为基于领域知识的概括性描述，具体研究请以实际文献内容为准。)
Epsin-2 (EPN2), a member of the epsin family, is a multifunctional adaptor protein involved in clathrin-mediated endocytosis and membrane remodeling processes. It plays a critical role in regulating receptor internalization, vesicle formation, and intracellular trafficking by interacting with clathrin, adaptor proteins, and phosphatidylinositol lipids. The N-terminal ENTH (epsin N-terminal homology) domain of EPN2 binds specifically to phosphatidylinositol-4.5-bisphosphate [PI(4.5)P₂] on the plasma membrane, promoting membrane curvature, while its C-terminal region recruits clathrin and other endocytic components. Dysregulation of EPN2 has been linked to neurological disorders, cancer progression, and cardiovascular diseases, highlighting its importance in cellular homeostasis.
Recombinant human EPN2 protein is engineered using heterologous expression systems (e.g., E. coli, mammalian cells) to produce functional, purified EPN2 for biomedical research. Its applications include studying endocytosis mechanisms, screening therapeutic agents targeting vesicular trafficking, and exploring pathological pathways in diseases associated with EPN2 dysfunction. The recombinant form retains key biochemical properties, enabling in vitro studies of protein-lipid interactions, structural analysis, and functional assays. This tool accelerates research into EPN2’s role in cellular physiology and its potential as a diagnostic or therapeutic target.
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