| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | EPC1 |
| Uniprot No | Q9H2F5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-813aa |
| 氨基酸序列 | MSKLSFRARALDASKPLPVFRCEDLPDLHEYASINRAVPQMPTGMEKEEESEHHLQRAISAQQVYGEKRDNMVIPVPEAESNIAYYESIYPGEFKMPKQLIHIQPFSLDAEQPDYDLDSEDEVFVNKLKKKMDICPLQFEEMIDRLEKGSGQQPVSLQEAKLLLKEDDELIREVYEYWIKKRKNCRGPSLIPSVKQEKRDGSSTNDPYVAFRRRTEKMQTRKNRKNDEASYEKMLKLRRDLSRAVTILEMIKRREKSKRELLHLTLEIMEKRYNLGDYNGEIMSEVMAQRQPMKPTYAIPIIPITNSSQFKHQEAMDVKEFKVNKQDKADLIRPKRKYEKKPKVLPSSAAATPQQTSPAALPVFNAKDLNQYDFPSSDEEPLSQVLSGSSEAEEDNDPDGPFAFRRKAGCQYYAPHLDQTGNWPWTSPKDGGLGDVRYRYCLTTLTVPQRCIGFARRRVGRGGRVLLDRAHSDYDSVFHHLDLEMLSSPQHSPVNQFANTSETNTSDKSFSKDLSQILVNIKSCRWRHFRPRTPSLHDSDNDELSCRKLYRSINRTGTAQPGTQTCSTSTQSKSSSGSAHFAFTAEQYQQHQQQLALMQKQQLAQIQQQQANSNSSTNTSQGFVSKTLDSASAQFAASALVTSEQLMGFKMKDDVVLGIGVNGVLPASGVYKGLHLSSTTPTALVHTSPSTAGSALLQPSNITQTSSSHSALSHQVTAANSATTQVLIGNNIRLTVPSSVATVNSIAPINARHIPRTLSVVPSSALKLAAAANCQVSKVPSSSSVDSVPRENHESEKPALNNIADNTVAMEVT |
| 分子量 | 117.3 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为示例性的参考文献，基于重组人EPC1蛋白的可能研究方向进行概括，建议通过学术数据库(如PubMed、Google Scholar)获取真实文献：
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1. **文献名称**: "Recombinant Human EPC1 Enhances Polycomb Repressive Complex 2-Mediated Gene Silencing"
**作者**: Smith, A.B., et al.
**摘要**: 研究报道了重组人EPC1蛋白在大肠杆菌中的表达与纯化，并证明其通过增强PRC2复合体的组蛋白H3K27三甲基化活性，调控靶基因的转录沉默。
2. **文献名称**: "EPC1 Promotes Endothelial Progenitor Cell Angiogenic Function via VEGF Signaling"
**作者**: Zhang, Y., et al.
**摘要**: 研究发现重组人EPC1蛋白可激活内皮祖细胞中的VEGF通路，促进血管生成和细胞迁移，为缺血性疾病治疗提供潜在策略。
3. **文献名称**: "Structural and Functional Analysis of EPC1 in Chromatin Remodeling Complexes"
**作者**: Lee, H., et al.
**摘要**: 利用重组人EPC1蛋白进行结构解析，揭示了其与染色质重塑复合体BAF的相互作用机制，及其在胚胎干细胞分化中的关键作用。
4. **文献名称**: "High-Yield Production of Recombinant EPC1 in Mammalian Cells and Its Anti-Tumor Activity"
**作者**: Wang, Q., et al.
**摘要**: 开发了哺乳动物细胞表达系统高效制备重组人EPC1蛋白，并发现其通过抑制癌基因MYC的转录，抑制乳腺癌细胞增殖。
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**注**：以上文献为模拟示例，实际研究中请查阅具体学术数据库获取真实信息。若需进一步协助检索，请提供更详细的研究背景或蛋白功能关键词。
Recombinant human EPC1 (enhancer of polycomb-like protein 1) is a protein engineered through DNA recombination technology for research and therapeutic applications. EPC1 belongs to the polycomb group (PcG) protein family, which plays critical roles in epigenetic regulation by modulating chromatin structure and gene expression. As a transcriptional regulator, EPC1 is implicated in maintaining cellular identity, differentiation, and development through interactions with Polycomb repressive complexes (PRCs). It contains conserved domains, such as the EPC homology domain, enabling binding to histones or other chromatin-associated proteins.
The recombinant form is typically produced in bacterial or mammalian expression systems, ensuring high purity and stability for experimental use. Studies highlight its involvement in DNA repair, cell cycle control, and tumor suppression, with dysregulation linked to cancers and developmental disorders. EPC1 also interacts with key signaling pathways, including Wnt and Notch, underscoring its multifaceted role in cellular homeostasis.
Research on recombinant EPC1 aids in elucidating its molecular mechanisms, such as histone modification (e.g., acetylation or methylation) and gene silencing. It serves as a tool for investigating epigenetic therapies, cancer biomarkers, and stem cell biology. Further exploration of EPC1’s structure-function relationships could advance targeted interventions for diseases tied to epigenetic dysregulation.
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