| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | EPB41L1 |
| Uniprot No | Q9H4G0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-779aa |
| 氨基酸序列 | MEEKDYSEADGLSERTTPSKAQKSPQKIAKKYKSAICRVTLLDASEYECEVEKHGRGQVLFDLVCEHLNLLEKDYFGLTFCDADSQKNWLDPSKEIKKQIRSSPWNFAFTVKFYPPDPAQLTEDITRYYLCLQLRADIITGRLPCSFVTHALLGSYAVQAELGDYDAEEHVGNYVSELRFAPNQTRELEERIMELHKTYRGMTPGEAEIHFLENAKKLSMYGVDLHHAKDSEGIDIMLGVCANGLLIYRDRLRINRFAWPKILKISYKRSNFYIKIRPGEYEQFESTIGFKLPNHRSAKRLWKVCIEHHTFFRLVSPEPPPKGFLVMGSKFRYSGRTQAQTRQASALIDRPAPFFERSSSKRYTMSRSLDGAEFSRPASVSENHDAGPDGDKRDEDGESGGQRSEAEEGEVRTPTKIKELKFLDKPEDVLLKHQASINELKRTLKEPNSKLIHRDRDWERERRLPSSPASPSPKGTPEKANERAGLREGSEEKVKPPRPRAPESDTGDEDQDQERDTVFLKDNHLAIERKCSSITVSSTSSLEAEVDFTVIGDYHGSAFEDFSRSLPELDRDKSDSDTEGLLFSRDLNKGAPSQDDESGGIEDSPDRGACSTPDMPQFEPVKTETMTVSSLAIRKKIEPEAVLQTRVSAMDNTQENSLKSGKGAAAMIPGPQTVATEIRSLSPIIGKDVLTSTYGATAETLSTSTTTHVTKTVKGGFSETRIEKRIIITGDEDVDQDQALALAIKEAKLQHPDMLVTKAVVYRETDPSPEERDKKPQES |
| 分子量 | 114 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人EPB41L1蛋白的虚构参考文献示例(仅供参考，实际文献请查阅学术数据库)：
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1. **标题**: "Expression and purification of recombinant human EPB41L1 and its interaction with cytoskeletal proteins"
**作者**: Smith J.R., Chen L., et al.
**摘要**: 本文报道了通过大肠杆菌系统高效表达重组人EPB41L1蛋白，并优化了纯化方法。研究发现，纯化的EPB41L1能与肌动蛋白和α-血影蛋白结合，提示其在细胞骨架动态调节中起关键作用。
2. **标题**: "Structural characterization of the FERM domain in EPB41L1 and its role in membrane-cytoskeletal linkage"
**作者**: Gonzalez M., Wei Y., et al.
**摘要**: 通过X射线晶体学解析了EPB41L1蛋白的FERM结构域三维结构，揭示了该结构域通过与细胞膜磷脂和整合素结合，介导细胞膜-细胞骨架连接的分子机制。
3. **标题**: "EPB41L1 suppresses tumor cell migration by regulating Rho GTPase signaling pathways"
**作者**: Tanaka K., Wang X., et al.
**摘要**: 研究显示，重组EPB41L1蛋白在多种癌细胞系中显著抑制迁移和侵袭能力。机制研究表明，其通过抑制RhoA/ROCK通路并激活Rac1.重塑细胞骨架结构及黏着斑动态。
4. **标题**: "Role of EPB41L1 in neuronal synapse formation: Insights from recombinant protein assays"
**作者**: Patel S., Li H., et al.
**摘要**: 利用重组EPB41L1体外实验发现，该蛋白通过结合突触后致密蛋白PSD-95.促进神经元突触形成及稳定性，为神经发育异常相关疾病提供潜在研究靶点。
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(注：以上为示例文献，实际研究中建议通过PubMed、Google Scholar等平台检索真实发表论文。)
Recombinant human EPB41L1 protein, also known as erythrocyte membrane protein band 4.1-like 1. is a cytoskeletal protein encoded by the EPB41L1 gene. It belongs to the protein 4.1 superfamily, which is characterized by a conserved FERM (Four-point-one, Ezrin, Radixin, Moesin) domain critical for membrane-cytoskeleton interactions. EPB41L1 plays a role in maintaining cell structure, regulating cell adhesion, and signaling pathways. It interacts with transmembrane proteins, cytoskeletal components (e.g., actin), and signaling molecules, influencing cellular processes like polarity, migration, and apoptosis.
The protein is widely expressed in tissues, particularly in the nervous system, where it supports neuronal development and synaptic stability. Dysregulation of EPB41L1 has been linked to cancer, as it may act as a tumor suppressor by inhibiting proliferation and metastasis. For instance, its downregulation is associated with malignancies like glioblastoma and lung cancer.
Recombinant EPB41L1 is produced using expression systems (e.g., E. coli, mammalian cells) to ensure high purity and activity for in vitro studies. Its production enables researchers to explore molecular interactions, structural-functional relationships, and therapeutic potential. Applications include investigating its role in tumor suppression, neuronal disorders, and cell mechanics. However, post-translational modifications in native tissues may differ from recombinant forms, necessitating careful experimental design.
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