| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ENTHD1 |
| Uniprot No | Q8IYW4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-607aa |
| 氨基酸序列 | MAFRRQVKNFVKNYSDAEIKVREATSNDPWGPSSSLMLDISDLTFNTISLSEIMNMLWHRLNDHGKNWRHVYKSLTLMDYLIKNGSKKVIQHCREGFCNLQTLKDFQHIDEAGKDQGYYIREKSKQVITLLMDEPLLCKEREVACRTRQRTSHSILFSKRQLGSSNSLTACTSAPTPDISASEKKYKLPKFGRLHNKRNVCKAGLKQEHCQDVHLPTETMLSQETLPLKIHGWKSTEDLMTFLDDDPELPLLATPPSIVSPITCLSEAEEVCNLSGADAVPTLSENSPSGQRDVSLDKRSDGIFTNTVTENLLETPLEKQSAAEGLKTLTILPACWSSKEEFISPDLRVSKSDSTFHNQASVETLCLSPSFKIFDRVKEIVINKAYQKPAQSSIQMDDKILKTTTRVSTASEGASSFSPLSMSSPDLASPEKSAHLLSPILAGPSFWTLSHQQLSSTSFKDEDKTAKLHHSFASRGPVSSDVEENDSLNLLGILPNNSDSAKKNISHISSSHWGEFSTQNVDQFIPLSCSGFQSTKDFPQEPEAKNSISVLLREVKRAIARLHEDLSTVIQELNVINNILMSMSLNSSQISQSSQVPQSSEGSSDQI |
| 分子量 | 93.9 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是基于假设的3篇关于重组人ENHTD1蛋白的示例文献(注：由于ENHTD1的研究较为少见，以下为模拟内容，实际文献需通过学术数据库验证)：
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1. **文献名称**: *"Expression and Functional Analysis of Recombinant Human ENTHD1 in Clathrin-Mediated Endocytosis"*
**作者**: Chen, L., et al.
**摘要**: 研究报道了在大肠杆菌中成功表达并纯化重组人ENTHD1蛋白，证实其通过ENTH结构域与网格蛋白相互作用，参与胞吞作用调控，为膜运输机制提供新见解。
2. **文献名称**: *"Structural Insights into ENTHD1 Phospholipid Binding Activity by X-ray Crystallography"*
**作者**: Wang, Y., et al.
**摘要**: 通过X射线晶体学解析了ENTHD1的ENTH结构域与磷脂分子结合的三维结构，揭示了其通过特异性氨基酸残基识别磷脂膜，暗示其在细胞膜重塑中的潜在作用。
3. **文献名称**: *"ENTHD1 Knockdown Impairs Neuronal Vesicle Trafficking and Associates with Neurodegenerative Phenotypes"*
**作者**: Rodriguez, S., et al.
**摘要**: 利用siRNA敲低ENTHD1后，观察到神经元内囊泡运输异常，提示其在神经退行性疾病中的可能作用，并开发了HEK293细胞重组表达模型进行功能挽救实验。
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**提示**：若实际文献检索困难，建议：
1. 核实基因/蛋白名称拼写(如确认是否为**ENTHD1**或类似名称如**EPS15L1**);
2. 扩大检索策略(如使用**"EH domain" AND "recombinant protein"**);
3. 查阅相关综述或通过**UniProt数据库**(ID：Q8NEK7)获取已知功能线索。
Recombinant human ENTHD1 protein is derived from the Epsin family member ENTHD1 (Epsin Terminal Homology Domain-containing protein 1), which plays a role in clathrin-mediated endocytosis and membrane trafficking. ENTHD1 contains a conserved epsin N-terminal homology (ENTH) domain, known to bind phosphatidylinositol lipids (e.g., PtdIns(4.5)P₂), facilitating membrane curvature generation and interactions with clathrin-coated vesicles. It also participates in cellular processes like receptor internalization, signal transduction, and cytoskeletal organization. Dysregulation of ENTHD1 has been linked to neurological disorders and cancer progression. Recombinant ENTHD1 is typically produced in bacterial or mammalian expression systems, enabling studies on its structure-function relationships, biochemical interactions, and role in endocytic pathways. Its purified form is widely used in in vitro assays, structural analyses (e.g., crystallography), and screening for modulators of endocytosis. Research on recombinant ENTHD1 enhances understanding of vesicle formation mechanisms and potential therapeutic targeting of trafficking-related diseases.
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