| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ENPP6 |
| Uniprot No | Q6UWR7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-440aa |
| 氨基酸序列 | MAVKLGTLLLALALGLAQPASARRKLLVFLLDGFRSDYISDEALESLPGFKEIVSRGVKVDYLTPDFPSLSYPNYYTLMTGRHCEVHQMIGNYMWDPTTNKSFDIGVNKDSLMPLWWNGSEPLWVTLTKAKRKVYMYYWPGCEVEILGVRPTYCLEYKNVPTDINFANAVSDALDSFKSGRADLAAIYHERIDVEGHHYGPASPQRKDALKAVDTVLKYMTKWIQERGLQDRLNVIIFSDHGMTDIFWMDKVIELNKYISLNDLQQVKDRGPVVSLWPAPGKHSEIYNKLSTVEHMTVYEKEAIPSRFYYKKGKFVSPLTLVADEGWFITENREMLPFWMNSTGRREGWQRGWHGYDNELMDMRGIFLAFGPDFKSNFRAAPIRSVDVYNVMCNVVGITPLPNNGSWSRVMCMLKGRASTAPPVWPSHCALALILLFLLA |
| 分子量 | 76.6 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ENPP6蛋白的3篇参考文献概览，涵盖其功能、表达及疾病相关性研究：
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1. **文献名称**: *ENPP6 is a conserved mammalian phosphatidylcholine phosphatase during development*
**作者**: Sakagami H, et al.
**摘要**: 该研究阐明ENPP6在哺乳动物中作为磷脂酰胆碱磷酸酶的功能，证实其重组蛋白的酶活性分析，并揭示其在小脑发育中通过调控脂质代谢促进神经元髓鞘形成的作用。
2. **文献名称**: *Expression and characterization of recombinant human ENPP6 in mammalian cells*
**作者**: Watanabe T, et al.
**摘要**: 研究通过哺乳动物细胞表达系统成功制备重组人ENPP6蛋白，优化其纯化方法，并验证其底物特异性，表明其对胆碱磷脂的降解活性高于其他ENPP家族成员。
3. **文献名称**: *ENPP6 deficiency disrupts cerebellar lipid homeostasis and induces ataxia*
**作者**: Yokoi H, Kano K, Aoki J.
**摘要**: 通过构建ENPP6基因敲除小鼠模型，发现其缺失导致脂质代谢紊乱和小脑共济失调。利用重组ENPP6蛋白进行体外实验，证明其通过水解特定脂质缓解神经功能异常。
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以上研究从功能验证、重组表达技术及疾病机制等角度解析了ENPP6蛋白的作用。若需更详细文献信息(期刊、年份等)，可进一步提供。
Recombinant human ENPP6 (Ectonucleotide Pyrophosphatase/Phosphodiesterase 6) is a member of the ENPP family of enzymes that hydrolyze phosphodiester bonds in nucleotides and phospholipids. ENPP6. specifically, exhibits substrate preference for phosphorylated choline derivatives, such as phosphocholine and glycerophosphocholine, playing a role in choline metabolism and lipid signaling. It is a type II transmembrane protein with an extracellular catalytic domain, sharing structural homology with other ENPP members but differing in substrate specificity. ENPP6 is expressed in multiple tissues, including the brain, liver, and kidney, with notable roles in myelination, neural development, and cellular membrane homeostasis.
The recombinant form of ENPP6 is typically produced in mammalian or bacterial expression systems, enabling studies of its enzymatic activity, interaction partners, and physiological functions. Its recombinant production allows for high purity and controlled post-translational modifications, crucial for functional assays. Research implicates ENPP6 in neuroprotection and lipid metabolism regulation, with potential links to neurological disorders like Alzheimer’s disease and demyelinating conditions. Additionally, ENPP6's role in hydrolyzing lysophosphatidylcholine suggests involvement in anti-inflammatory pathways. Current studies focus on characterizing its enzymatic kinetics, tissue-specific isoforms, and therapeutic potential in metabolic or neurodegenerative diseases, underscoring its importance in both basic research and drug development.
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