| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ENC2 |
| Uniprot No | Q9H0H3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-589aa |
| 氨基酸序列 | MSVSVHETRKSRSSTGSMNVTLFHKASHPDCVLAHLNTLRKHCMFTDVTLWAGDRAFPCHRAVLAASSRYFEAMFSHGLRESRDDTVNFQDNLHPEVLELLLDFAYSSRIAINEENAESLLEAGDMLQFHDVRDAAAEFLEKNLFPSNCLGMMLLSDAHQCRRLYEFSWRMCLVHFETVRQSEDFNSLSKDTLLDLISSDELETEDERVVFEAILQWVKHDLEPRKVHLPELLRSVRLALLPSDCLQEAVSSEALLMADERTKLIMDEALRCKTRILQNDGVVTSPCARPRKAGHTLLILGGQTFMCDKIYQVDHKAKEIIPKADLPSPRKEFSASAIGCKVYVTGGRGSENGVSKDVWVYDTVHEEWSKAAPMLIARFGHGSAELENCLYVVGGHTSLAGVFPASPSVSLKQVEKYDPGANKWMMVAPLRDGVSNAAVVSAKLKLFVFGGTSIHRDMVSKVQCYDPSENRWTIKAECPQPWRYTAAAVLGSQIFIMGGDTEFTAASAYRFDCETNQWTRIGDMTAKRMSCHALASGNKLYVVGGYFGTQRCKTLDCYDPTSDTWNCITTVPYSLIPTAFVSTWKHLPA |
| 分子量 | 90.64 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ENC2蛋白的虚构参考文献示例(注：实际文献需通过学术数据库验证)：
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1. **文献名称**: *"Expression and Functional Characterization of Recombinant Human ENC2 in Epigenetic Regulation"*
**作者**: Smith, J. et al.
**摘要**: 本研究成功表达并纯化了重组人ENC2蛋白，证明其作为染色质修饰因子的功能。实验显示，ENC2通过结合组蛋白H3K27位点调控基因沉默，为癌症表观遗传学研究提供新视角。
2. **文献名称**: *"Structural Insights into the ENC2 Protein: Implications for Neural Differentiation"*
**作者**: Li, X. & Wang, Y.
**摘要**: 通过X射线晶体学解析重组人ENC2蛋白的三维结构，揭示其C端结构域参与神经干细胞分化的分子机制，提示其在神经发育疾病中的潜在作用。
3. **文献名称**: *"High-Yield Production of Recombinant Human ENC2 in E. coli and Its Application in Drug Screening"*
**作者**: Chen, R. et al.
**摘要**: 开发了一种高效的大肠杆菌表达系统，规模化生产具有生物活性的重组ENC2蛋白，并用于筛选靶向表观遗传通路的小分子抑制剂。
4. **文献名称**: *"ENC2 Knockdown and Rescue Models Reveal Its Role in Tumor Suppression"*
**作者**: Hirotsune, S. et al.
**摘要**: 利用重组ENC2蛋白在细胞模型中恢复功能，证实其在抑制结直肠癌细胞增殖中的作用，并揭示其通过p53通路调控细胞周期的机制。
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如需真实文献，建议通过PubMed或Google Scholar检索关键词“hENC2 recombinant”“ENC2 protein function”等，并筛选近年研究。
Recombinant human ENC2 (eukaryotic translation initiation factor 4E nuclear import factor 2) is a protein encoded by the EIF4ENIF1 gene, primarily involved in regulating mRNA transport and translation. It plays a role in shuttling the translation initiation factor eIF4E into the nucleus, influencing gene expression by modulating mRNA availability. ENC2 contains a conserved eIF4E-binding domain and nuclear localization signals, enabling interaction with eIF4E and participation in nucleocytoplasmic trafficking. Dysregulation of ENC2 has been linked to cancers, as altered mRNA export/translation can promote oncogenesis. Recombinant ENC2 is typically produced in bacterial or mammalian expression systems for functional studies. Its applications include investigating mRNA metabolism, nuclear transport mechanisms, and eIF4E-related pathways. Recent studies also explore its role in cellular stress responses and potential as a therapeutic target. The recombinant protein serves as a tool to dissect its structure-function relationships and interactions with eIF4E-family proteins. Ongoing research aims to clarify its tissue-specific roles and implications in diseases like leukemia or solid tumors.
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