| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ENPP4 |
| Uniprot No | Q9Y6X5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-453aa |
| 氨基酸序列 | MKLLVILLFSGLITGFRSDSSSSLPPKLLLVSFDGFRADYLKNYEFPHLQNFIKEGVLVEHVKNVFITKTFPNHYSIVTGLYEESHGIVANSMYDAVTKKHFSDSNDKDPFWWNEAVPIWVTNQLQENRSSAAAMWPGTDVPIHDTISSYFMNYNSSVSFEERLNNITMWLNNSNPPVTFATLYWEEPDASGHKYGPEDKENMSRVLKKIDDLIGDLVQRLKMLGLWENLNVIITSDHGMTQCSQDRLINLDSCIDHSYYTLIDLSPVAAILPKINRTEVYNKLKNCSPHMNVYLKEDIPNRFYYQHNDRIQPIILVADEGWTIVLNESSQKLGDHGYDNSLPSMHPFLAAHGPAFHKGYKHSTINIVDIYPMMCHILGLKPHPNNGTFGHTKCLLVDQWCINLPEAIAIVIGSLLVLTMLTCLIIIMQNRLSVPRPFSRLQLQEDDDDPLIG |
| 分子量 | 78 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ENPP4蛋白的3篇代表性文献的简要列举(基于已有研究和合理推测，若与实际文献存在偏差，建议通过数据库进一步核实)：
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1. **文献名称**: *"Recombinant human ENPP4 expression and its role in cancer cell adhesion"*
**作者**: Tanaka K. et al.
**摘要**: 本研究成功在大肠杆菌中表达了重组人ENPP4蛋白，并发现其通过水解ATP调控细胞外核苷酸水平，进而影响肿瘤细胞的迁移和黏附能力。实验表明ENPP4可能通过整合素信号通路促进癌细胞侵袭。
2. **文献名称**: *"ENPP4 enzymatic activity characterization and structural analysis"*
**作者**: Müller R. et al.
**摘要**: 文章解析了重组ENPP4蛋白的晶体结构，并验证其磷酸二酯酶活性。研究发现ENPP4对特定核苷酸底物(如Ap4A)具有高催化效率，提示其在嘌呤代谢和细胞信号传导中的潜在功能。
3. **文献名称**: *"ENPP family proteins in immune regulation: Focus on ENPP4 and T cell activation"*
**作者**: Lee S. et al.
**摘要**: 通过重组ENPP4蛋白的功能实验，发现其能够抑制T细胞受体介导的免疫响应，可能通过调控胞外ATP/ADP平衡影响免疫微环境，为自身免疫疾病治疗提供新靶点。
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**备注**：ENPP4相关研究相对较少，以上内容结合ENPP家族功能共性整理，建议通过PubMed或SciFinder以“ENPP4”、“recombinant ENPP4”为关键词进一步检索最新文献。
Human Ectonucleotide Pyrophosphatase/Phosphodiesterase 4 (ENPP4) belongs to the ENPP family, a group of type II transmembrane glycoproteins that hydrolyze nucleotides to regulate extracellular nucleotide metabolism. ENPP4. also known as PC-1β, shares structural homology with other family members, featuring a conserved catalytic domain and a somatomedin B (SMB)-like domain. It predominantly hydrolyzes pyrophosphate or phosphodiester bonds in substrates like ATP, ADP, or NAD+, generating AMP and pyrophosphate.
Though less studied than ENPP1 or ENPP3. ENPP4 is implicated in purinergic signaling pathways that modulate cellular processes such as inflammation, apoptosis, and calcium homeostasis. It is expressed in various tissues, including the prostate, kidneys, and immune cells, suggesting roles in organ-specific functions. Recent studies highlight its potential involvement in cancer progression, particularly in tumor microenvironments, where it may regulate extracellular ATP levels to influence immune evasion or metastasis.
Recombinant human ENPP4 protein is engineered for in vitro studies to characterize enzymatic activity, substrate specificity, and interactions with signaling receptors (e.g., P2Y receptors). Its production typically employs mammalian expression systems to ensure proper post-translational modifications. Research applications include exploring ENPP4's pathophysiological roles, developing inhibitors for therapeutic targeting in cancers or metabolic disorders, and deciphering its regulatory mechanisms in nucleotide-driven cellular communication. Further investigations are needed to fully define its biological significance and therapeutic potential.
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