| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ENDOD1 |
| Uniprot No | O94919 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-500aa |
| 氨基酸序列 | MGTARWLALGSLFALAGLLEGRLVGEEEAGFGECDKFFYAGTPPAGLAADSHVKICQRAEGAERFATLYSTRDRIPVYSAFRAPRPAPGGAEQRWLVEPQIDDPNSNLEEAINEAEAITSVNSLGSKQALNTDYLDSDYQRGQLYPFSLSSDVQVATFTLTNSAPMTQSFQERWYVNLHSLMDRALTPQCGSGEDLYILTGTVPSDYRVKDKVAVPEFVWLAACCAVPGGGWAMGFVKHTRDSDIIEDVMVKDLQKLLPFNPQLFQNNCGETEQDTEKMKKILEVVNQIQDEERMVQSQKSSSPLSSTRSKRSTLLPPEASEGSSSFLGKLMGFIATPFIKLFQLIYYLVVAILKNIVYFLWCVTKQVINGIESCLYRLGSATISYFMAIGEELVSIPWKVLKVVAKVIRALLRILCCLLKAICRVLSIPVRVLVDVATFPVYTMGAIPIVCKDIALGLGGTVSLLFDTAFGTLGGLFQVVFSVCKRIGYKVTFDNSGEL |
| 分子量 | 81.4 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ENDOD1蛋白的模拟参考文献示例(供参考，非真实文献)：
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1. **文献名称**：Expression and Characterization of Recombinant Human ENDOD1 in Mammalian Cells
**作者**：Smith A, et al.
**摘要**：本研究利用HEK293细胞系统成功表达并纯化重组人ENDOD1蛋白，证实其具有核酸内切酶活性，并揭示其在DNA损伤修复中的潜在作用。
2. **文献名称**：Structural Insights into ENDOD1’s Role in Genome Stability
**作者**：Zhang L, et al.
**摘要**：通过X射线晶体学解析了重组人ENDOD1蛋白的三维结构，结合功能实验表明其通过结合特定DNA序列调控基因组稳定性，为癌症治疗提供新靶点。
3. **文献名称**：Functional Analysis of ENDOD1 in Apoptotic Signaling Pathways
**作者**：Kim Y, et al.
**摘要**：利用重组ENDOD1蛋白进行体外实验，发现其通过激活caspase通路促进细胞凋亡，提示其在神经退行性疾病中的功能关联。
4. **文献名称**：Optimization of ENDOD1 Protein Production in E. coli for Therapeutic Applications
**作者**：Wang Q, et al.
**摘要**：开发了一种高效的大肠杆菌表达系统，用于大规模生产可溶性重组人ENDOD1.并验证其在基因编辑工具开发中的应用潜力。
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**注意**：以上内容为模拟文献摘要，实际研究中需通过学术数据库(如PubMed、Web of Science)检索真实文献。若需进一步协助定位真实论文，请提供更详细的背景信息。
Recombinant human ENDOD1 (Endonuclease Domain-containing 1) protein is a engineered version of a naturally occurring human enzyme involved in nucleic acid metabolism. ENDOD1. also known as Endonuclease G-like 1 (ENDOGL1), belongs to the DNA/RNA non-specific endonuclease family. It contains conserved endonuclease catalytic domains critical for cleaving single-stranded RNA and DNA substrates.
Native ENDOD1 plays roles in mitochondrial DNA repair, RNA processing, and maintaining genomic stability. Studies suggest its involvement in apoptosis, viral defense, and cell cycle regulation. The recombinant form is typically produced in Escherichia coli or mammalian expression systems with tags (e.g., His-tag) for simplified purification. This engineered protein retains enzymatic activity while enabling standardized applications in molecular research.
Current research focuses on characterizing ENDOD1’s substrate specificity, interaction partners, and precise biological functions. Its recombinant version serves as a vital tool for in vitro studies on nucleic acid degradation mechanisms, mitochondrial dysfunction-related diseases, and potential therapeutic targeting. Recent investigations also explore its role in innate immunity and association with cancer progression. However, complete functional insights remain limited, necessitating further structural and mechanistic studies.
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