| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | EP400NL |
| Uniprot No | Q6ZTU2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-419aa |
| 氨基酸序列 | MQHVSSSQSSQRHVQWPGACPGAGEEQPACSQPSLPLTLPSPSHQLQQLMVQTQSPTQPSPGPGQALQNVRAGAPGPGLGLCSSSPTGDFVDASVLVRQISLSPSSGGHFVFQDGSGLTQIAQGAQVQLQHPGTPITVRERRPSQPHTQSGGTIHHLGPQSPAAAGGAGLQPLASPSHITTANLPPQISSIIQGQLVQQQQVLQGPPLPRPLGFERTPGVLLPGAGGAAGFGMTSPPPPTSPSRTAVPPGLSSLPLTSVGNTGMKKVPKKLEEIPPASPEMAQMRKQCLDYHHQEMQALKEVFKEYLIELFFLQHFQGNMMDFLAFKERLYGPLQAYLRQNDLDIEEEEEEHFEVINDEVKVVARKHGQPGTPVAIATQLPPRTSAAFPAQQQPLQVLSDGSTVQLPRLSSLGFEDSMC |
| 分子量 | 71 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人EP400NL蛋白的3篇模拟参考文献示例(注：EP400NL相关研究较少，部分文献可能涉及相近蛋白或复合物功能)：
---
1. **文献名称**：**"Characterization of the EP400NL Chromatin Remodeling Complex in DNA Repair"**
**作者**：Thompson, R. et al.
**摘要**：本研究解析了重组人EP400NL蛋白在DNA损伤修复中的作用，证实其与TIP60组蛋白乙酰转移酶复合物结合，通过染色质重塑调控DNA双链断裂修复。通过体外表达重组蛋白，发现其依赖ATP酶活性参与染色质结构动态变化。
2. **文献名称**：**"Recombinant EP400NL Expression and Its Role in Transcriptional Regulation"**
**作者**：Lee, S. & Martinez, E.
**摘要**：作者成功在大肠杆菌中表达并纯化了重组人EP400NL蛋白，功能实验表明其通过调控组蛋白H2A.Z的交换影响基因启动子区域的可及性，揭示了其在胚胎干细胞分化中的表观遗传调控机制。
3. **文献名称**：**"Structural Analysis of the p400/EP400NL Complex in Chromatin Remodeling"**
**作者**：Kurokawa, Y. et al.
**摘要**：通过冷冻电镜技术解析了包含重组EP400NL蛋白的p400复合物结构，阐明其通过识别特定组蛋白修饰(如H3K4me3)靶向染色质区域，并利用ATP水解活性重塑核小体构象的分子机制。
---
**备注**：上述文献为示例，实际研究中关于EP400NL的重组表达和功能的文献较少，建议通过PubMed或Google Scholar结合关键词(如“EP400NL recombinant”、“p400 complex”)进一步检索。
The recombinant human EP400NL protein is a truncated, engineered variant derived from the EP400 protein, a large multi-domain enzyme belonging to the SWI/SNF2 family of chromatin remodelers. EP400. also known as p400 or TIP60-associated chromatin remodeling enzyme, plays crucial roles in DNA repair, transcriptional regulation, and chromatin structure modulation by utilizing ATP to reposition nucleosomes. The EP400NL variant lacks the N-terminal region of the full-length EP400 but retains functional domains critical for interactions with histone modifiers (e.g., TIP60 complex) and chromatin-associated proteins. Its recombinant form is commonly expressed in vitro for structural and functional studies to dissect EP400's role in chromatin dynamics without interference from the full protein's size or complexity. Research on EP400NL has provided insights into how chromatin remodeling fine-tunes gene expression, particularly in pathways involving oncogenesis and cellular differentiation. Additionally, it serves as a tool to study molecular mechanisms in diseases linked to epigenetic dysregulation, such as cancers or neurodevelopmental disorders. The protein’s solubility and stability in recombinant form facilitate biochemical assays, crystallography, and drug screening targeting chromatin remodeling pathways.
×
