| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ENTPD4 |
| Uniprot No | Q9Y227 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-571aa |
| 氨基酸序列 | MGRIGISCLFPASWHFSISPVGCPRILNTNLRQIMVISVLAAAVSLLYFSVVIIRNKYGRLTRDKKFQRYLARVTDIEATDTNNPNVNYGIVVDCGSSGSRVFVYCWPRHNGNPHDLLDIRQMRDKNRKPVVMKIKPGISEFATSPEKVSDYISPLLNFAAEHVPRAKHKETPLYILCTAGMRILPESQQKAILEDLLTDIPVHFDFLFSDSHAEVISGKQEGVYAWIGINFVLGRFEHIEDDDEAVVEVNIPGSESSEAIVRKRTAGILDMGGVSTQIAYEVPKTEEVAKNLLAEFNLGCDVHQTEHVYRVYVATFLGFGGNAARQRYEDRIFANTIQKNRLLGKQTGLTPDMPYLDPCLPLDIKDEIQQNGQTIYLRGTGDFDLCRETIQPFMNKTNETQTSLNGVYQPPIHFQNSEFYGFSEFYYCTEDVLRMGGDYNAAKFTKAAKDYCATKWSILRERFDRGLYASHADLHRLKYQCFKSAWMFEVFHRGFSFPVNYKSLKTALQVYDKEVQWTLGAILYRTRFLPLRKIGMLIIPCSGGCPKLCCAVWQPPPYGATKHLQCAAWN |
| 分子量 | 91.3 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ENTPD4蛋白的参考文献示例(注：以下内容为示例，实际文献需根据研究领域具体检索)：
1. **文献名称**: *"Cloning, expression, and characterization of human ENTPD4: a novel ATP-diphosphohydrolase involved in extracellular nucleotide metabolism"*
**作者**: Smith A, et al.
**摘要**: 本研究成功克隆了人ENTPD4基因，并在哺乳动物表达系统中重组表达纯化该蛋白。通过酶活分析发现，ENTPD4特异性水解ATP/ADP，提示其在胞外核苷酸代谢及嘌呤信号通路中的潜在调控作用。
2. **文献名称**: *"Structural insights into the catalytic mechanism of ENTPD4 through X-ray crystallography"*
**作者**: Chen L, et al.
**摘要**: 通过X射线晶体学解析了重组人ENTPD4蛋白的三维结构，揭示其底物结合域的关键氨基酸残基，并阐明了其催化核苷酸水解的分子机制。
3. **文献名称**: *"Functional analysis of ENTPD4 in cancer cell migration via regulation of extracellular ATP signaling"*
**作者**: Wang Y, et al.
**摘要**: 研究发现重组ENTPD4通过降解肿瘤微环境中的ATP，抑制P2受体介导的癌细胞迁移和侵袭，表明其在肿瘤转移中的潜在干预价值。
4. **文献名称**: *"Optimization of recombinant ENTPD4 production in E. coli for enzymatic activity assays"*
**作者**: Johnson R, et al.
**摘要**: 开发了一种高效的大肠杆菌表达系统用于ENTPD4重组蛋白的可溶性表达，并通过层析技术优化纯化步骤，为大规模酶活筛选及药物开发提供基础。
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**注意事项**：
- ENTPD4相关研究相对较少，上述文献为综合示例，实际文献可能需要从专业数据库(如PubMed)以"ENTPD4"或"CANT1"(其别名)为关键词检索。
- 若需要具体文献，建议补充研究背景(如疾病相关、结构生物学等)以进一步筛选。
Recombinant human ENTPD4 protein is a genetically engineered form of the ectonucleoside triphosphate diphosphohydrolase 4 enzyme, which belongs to the ENTPDase family. ENTPD4. also known as CD39L3. is a membrane-associated glycoprotein involved in purinergic signaling by hydrolyzing extracellular nucleoside triphosphates (NTPs) and diphosphates (NDPs) into monophosphates. This enzymatic activity regulates the levels of ATP, ADP, and other nucleotides, modulating P2 receptor-mediated signaling pathways critical for inflammation, immune responses, and vascular function.
Structurally, ENTPD4 contains two apyrase-conserved regions (ACRs) responsible for substrate binding and catalysis. Unlike other ENTPDases (e.g., CD39/ENTPD1), ENTPD4 exhibits substrate specificity toward UDP and GTP, influencing distinct cellular processes such as glycogen metabolism and G-protein-coupled receptor signaling. Recombinant ENTPD4 is typically expressed in mammalian or insect cell systems to ensure proper post-translational modifications and enzymatic activity.
Research on recombinant ENTPD4 focuses on its role in pathological conditions, including cancer progression, neuroinflammation, and thrombosis. It serves as a tool to study nucleotide metabolism dysregulation and to screen therapeutic agents targeting purinergic pathways. Additionally, its interaction with immune cells highlights potential applications in immunotherapy. As an emerging target, ENTPD4's unique substrate profile and tissue-specific expression offer insights into developing precision therapies for diseases linked to purinergic signaling imbalances.
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