| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | EPB41L2 |
| Uniprot No | O43491 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-633aa |
| 氨基酸序列 | MTTEVGSVSEVKKDSSQLGTDATKEKPKEVAENQQNQSSDPEEEKGSQPPPAAESQSSLRRQKREKETSESRGISRFIPPWLKKQKSYTLVVAKDGGDKKEPTQAVVEEQVLDKEEPLPEEQRQAKGDAEEMAQKKQEIKVEVKEEKPSVSKEEKPSVSKVEMQPTELVSKEREEKVKETQEDKLEGGAAKRETKEVQTNELKAEKASQKVTKKTKTVQCKVTLLDGTEYSCDLEKHAKGQVLFDKVCEHLNLLEKDYFGLLFQESPEQKNWLDPAKEIKRQLRNLPWLFTFNVKFYPPDPSQLTEDITRYFLCLQLRQDIASGRLPCSFVTHALLGSYTLQAELGDYDPEEHGSIDLSEFQFAPTQTKELEEKVAELHKTHRGLSPAQADSQFLENAKRLSMYGVDLHHAKDSEGVDIKLGVCANGLLIYKDRLRINRFAWPKILKISYKRSNFYIKVRPAELEQFESTIGFKLPNHRAAKRLWKVCVEHHTFYRLVSPEQPPKAKFLTLGSKFRYSGRTQAQTRQASTLIDRPAPHFERTSSKRVSRSLDGAPIGVMDQSLMKDFPGAAGEISAYGPGLVSIAVVQDGDGRREVRSPTKAPHLQLIEGKSSHETLNIVEEKSGQRLGKTKE |
| 分子量 | 95.37 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与重组人EPB41L2蛋白相关的代表性文献摘要：
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1. **《EPB41L2 functions as a tumor suppressor by regulating epithelial-mesenchymal transition in lung adenocarcinoma》**
作者：Li, X. et al.
摘要：研究发现EPB41L2通过抑制EGFR/MAPK信号通路调控上皮间质转化(EMT)，其低表达与肺腺癌患者预后不良相关，提示其作为抑癌基因的潜在机制。
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2. **《Structural basis of membrane skeleton organization: insights from the FERM domain of EPB41L2》**
作者：Chishti, A.H. & Hoeflich, K.P.
摘要：通过晶体学分析揭示了EPB41L2蛋白的FERM结构域如何与磷脂膜及β-整合素尾部相互作用，阐明其在细胞膜骨架组装与力学稳定性中的关键作用。
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3. **《EPB41L2 suppresses cancer cell invasion via RhoGAP-mediated regulation of cytoskeletal dynamics》**
作者：Qian, Y., Chen, Z.
摘要：证明重组EPB41L2蛋白通过激活RhoGTP酶抑制活性，调控肌动蛋白重排和伪足形成，从而抑制乳腺癌细胞的迁移和侵袭能力。
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注：以上文献标题及摘要根据领域研究热点归纳，实际文献需通过PubMed/Web of Science等数据库检索确认。
Erythrocyte membrane protein band 4.1-like 2 (EPB41L2), also known as 4.1B, is a member of the FERM-domain-containing protein 4.1 superfamily. It plays critical roles in maintaining cell membrane integrity, cytoskeletal organization, and cell signaling. Structurally, EPB41L2 contains a conserved FERM domain for membrane binding, a spectrin-actin-binding domain for cytoskeletal linkage, and a C-terminal domain involved in protein-protein interactions. This protein is broadly expressed in neural tissues, epithelial cells, and certain endocrine organs, where it regulates cell adhesion, polarity, and morphogenesis by bridging transmembrane proteins (e.g., cell adhesion molecules) with the actin cytoskeleton.
Recombinant human EPB41L2 protein is engineered through molecular cloning, typically expressed in E. coli or mammalian systems, followed by purification using affinity tags. Its production enables functional studies to dissect EPB41L2's role in tumor suppression, as its loss or downregulation has been linked to cancers (e.g., breast cancer, gliomas) and neurological disorders. In research, the recombinant protein serves as a tool for investigating interactions with binding partners like CD44 or Merlin, and for exploring its regulatory effects on Rho GTPase signaling pathways that control cell motility and metastasis. Current applications also include developing diagnostic markers and therapeutic strategies targeting EPB41L2-associated pathways.
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