| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | EPS15L1 |
| Uniprot No | Q9UBC2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-864aa |
| 氨基酸序列 | MAAPLIPLSQQIPTGNSLYESYYKQVDPAYTGRVGASEAALFLKKSGLSDIILGKIWDLADPEGKGFLDKQGFYVALRLVACAQSGHEVTLSNLNLSMPPPKFHDTSSPLMVTPPSAEAHWAVRVEEKAKFDGIFESLLPINGLLSGDKVKPVLMNSKLPLDVLGRVWDLSDIDKDGHLDRDEFAVAMHLVYRALEKEPVPSALPPSLIPPSKRKKTVFPGAVPVLPASPPPKDSLRSTPSHGSVSSLNSTGSLSPKHSLKQTQPTVNWVVPVADKMRFDEIFLKTDLDLDGYVSGQEVKEIFMHSGLTQNLLAHIWALADTRQTGKLSKDQFALAMYFIQQKVSKGIDPPQVLSPDMVPPSERGTPGPDSSGSLGSGEFTGVKELDDISQEIAQLQREKYSLEQDIREKEEAIRQKTSEVQELQNDLDRETSSLQELEAQKQDAQDRLDEMDQQKAKLRDMLSDVRQKCQDETQMISSLKTQIQSQESDLKSQEDDLNRAKSELNRLQQEETQLEQSIQAGRVQLETIIKSLKSTQDEINQARSKLSQLHESRQEAHRSLEQYDQVLDGAHGASLTDLANLSEGVSLAERGSFGAMDDPFKNKALLFSNNTQELHPDPFQTEDPFKSDPFKGADPFKGDPFQNDPFAEQQTTSTDPFGGDPFKESDPFRGSATDDFFKKQTKNDPFTSDPFTKNPSLPSKLDPFESSDPFSSSSVSSKGSDPFGTLDPFGSGSFNSAEGFADFSQMSKPPPSGPFTSSLGGAGFSDDPFKSKQDTPALPPKKPAPPRPKPPSGKSTPVSQLGSADFPEAPDPFQPLGADSGDPFQSKKGFGDPFSGKDPFVPSSAAKPSKASASGFADFTSVS |
| 分子量 | 94.2 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人EPS15L1蛋白的虚构参考文献示例(实际文献需根据具体研究补充)：
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1. **"Recombinant human EPS15L1 regulates clathrin-mediated endocytosis in cancer cells"**
*Authors: Chen L, Wang X, Zhang Y.*
摘要：研究通过表达重组人EPS15L1蛋白，揭示其通过调控网格蛋白依赖的内吞作用影响表皮生长因子受体(EGFR)的胞内运输，从而抑制肺癌细胞增殖的机制。
2. **"Structural analysis of EPS15L1 reveals functional domains for protein interaction"**
*Authors: Müller S, Schmidt H, Braus GH.*
摘要：利用重组EPS15L1蛋白的晶体结构解析，鉴定出其EH结构域与泛素结合位点，为解释其在囊泡运输和肿瘤发生中的双重作用提供结构基础。
3. **"EPS15L1 modulates synaptic vesicle recycling in neurodegenerative models"**
*Authors: Park J, Kim T, Lee S.*
摘要：通过重组EPS15L1蛋白体外实验，证明其参与调节神经元突触囊泡回收过程，并与α-突触核蛋白异常聚集存在关联，可能成为阿尔茨海默病的干预靶点。
4. **"High-throughput screening of EPS15L1-binding partners using recombinant protein pulldown assays"**
*Authors: Gupta R, Patel K, Mishra A.*
摘要：建立基于重组EPS15L1的亲和纯化-质谱技术，筛选出多个内吞相关蛋白互作网络，并验证其在细胞迁移和侵袭中的功能。
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注：以上内容为示例，实际文献需通过 **PubMed/Google Scholar** 以关键词 *"recombinant EPS15L1"* 或 *"EPS15L1 protein function"* 检索，并核实具体研究内容。
Recombinant human EPS15L1 (Epidermal Growth Factor Receptor Pathway Substrate 15-Like 1) is a protein encoded by the EPS15L1 gene, which belongs to the EPS15 homology (EH) domain-containing protein family. EPS15L1 shares structural and functional similarities with its paralog EPS15. both involved in clathrin-mediated endocytosis, intracellular trafficking, and signal transduction. The protein contains three N-terminal EH domains that mediate interactions with proteins containing Asn-Pro-Phe (NPF) motifs, along with coiled-coil regions and a C-terminal domain associated with ubiquitin-binding activity. EPS15L1 plays a role in coordinating membrane dynamics by linking the endocytic machinery, such as adaptor proteins like Epsin and AP-2. to receptor tyrosine kinases (e.g., EGFR) and signaling pathways like Notch. Dysregulation of EPS15L1 has been implicated in cancer progression, particularly in regulating receptor internalization and downstream oncogenic signaling. Recombinant EPS15L1 protein, typically expressed in bacterial or mammalian systems using genetic engineering techniques, enables in vitro studies to dissect its molecular interactions, structural properties, and functional roles in cellular processes. Its purified form is widely used in binding assays, structural analyses, and pathway modulation experiments, serving as a critical tool for exploring therapeutic targets in endocytosis-related diseases.
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