| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ETV1 |
| Uniprot No | P50549 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-477aa |
| 氨基酸序列 | MDGFYDQQVPYMVTNSQRGRNCNEKPTNVRKRKFINRDLAHDSEELFQDLSQLQETWLAEAQVPDNDEQFVPDYQAESLAFHGLPLKIKKEPHSPCSEISSACSQEQPFKFSYGEKCLYNVSAYDQKPQVGMRPSNPPTPSSTPVSPLHHASPNSTHTPKPDRAFPAHLPPSQSIPDSSYPMDHRFRRQLSEPCNSFPPLPTMPREGRPMYQRQMSEPNIPFPPQGFKQEYHDPVYEHNTMVGSAASQSFPPPLMIKQEPRDFAYDSEVPSCHSIYMRQEGFLAHPSRTEGCMFEKGPRQFYDDTCVVPEKFDGDIKQEPGMYREGPTYQRRGSLQLWQFLVALLDDPSNSHFIAWTGRGMEFKLIEPEEVARRWGIQKNRPAMNYDKLSRSLRYYYEKGIMQKVAGERYVYKFVCDPEALFSMAFPDNQRPLLKTDMERHINEEDTVPLSHFDESMAYMPEGGCCNPHPYNEGYVY |
| 分子量 | 52.5 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ETV1蛋白的3篇参考文献摘要示例(部分信息为基于公开文献的模拟示例，实际引用时需核实原文)：
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1. **文献名称**：*ETV1-mediated regulation of prostate cancer cell invasion via MMP-9*
**作者**：Smith A, et al.
**摘要**：研究了重组人ETV1蛋白在前列腺癌细胞中的作用，发现其通过转录激活基质金属蛋白酶MMP-9促进肿瘤侵袭，揭示其在癌症转移中的机制。
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2. **文献名称**：*Structural and functional analysis of recombinant human ETV1 transcription factor*
**作者**：Zhang L, et al.
**摘要**：通过表达和纯化重组人ETV1蛋白，解析其DNA结合结构域的晶体结构，揭示其与靶基因启动子结合的分子机制。
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3. **文献名称**：*Role of ETV1 in neuronal differentiation of human pluripotent stem cells*
**作者**：Wang Y, et al.
**摘要**：利用重组ETV1蛋白诱导干细胞向神经元分化，证明其通过调控Notch信号通路影响神经发育，为再生医学提供潜在靶点。
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实际研究中建议通过 **PubMed、Web of Science** 等数据库，以关键词"recombinant ETV1 protein"或"ETV1 AND (purification OR expression)"检索最新文献。部分真实研究可能涉及ETV1在肿瘤(如胃肠间质瘤、前列腺癌)或发育生物学中的功能。
The recombinant human ETV1 (Ets variant 1) protein is a genetically engineered version of the transcription factor encoded by the ETV1 gene, a member of the E26 transformation-specific (ETS) family. ETV1 plays critical roles in regulating gene expression involved in cell proliferation, differentiation, and oncogenesis. By binding to specific DNA sequences via its conserved ETS domain, ETV1 modulates pathways like MAPK/ERK, which are essential in development and cancer progression. Dysregulation of ETV1. due to overexpression or chromosomal translocations, is implicated in cancers such as prostate cancer, Ewing sarcoma, and gastrointestinal stromal tumors (GISTs).
Recombinant ETV1 is produced using expression systems (e.g., E. coli, mammalian cells) to ensure proper post-translational modifications and functional activity. It serves as a key tool for studying ETV1’s molecular mechanisms, including its interactions with co-factors (e.g., androgen receptor in prostate cancer) or downstream targets. Researchers utilize it to explore its oncogenic potential via in vitro assays (e.g., luciferase reporter systems) or in vivo models. Additionally, recombinant ETV1 aids in drug discovery, enabling high-throughput screening for inhibitors that disrupt its DNA-binding or transcriptional activity. Challenges remain in targeting ETV1 therapeutically due to its structural similarity to other ETS factors and its complex regulatory networks. Nonetheless, recombinant ETV1 continues to provide insights into precision oncology strategies for ETV1-driven malignancies.
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