| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ETV3 |
| Uniprot No | P41162 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-512aa |
| 氨基酸序列 | MKAGCSIVEK PEGGGGYQFP DWAYKTESSP GSRQIQLWHF ILELLQKEEF RHVIAWQQGE YGEFVIKDPD EVARLWGRRK CKPQMNYDKL SRALRYYYNK RILHKTKGKR FTYKFNFNKL VMPNYPFINI RSSGVVPQSA PPVPTASSRF HFPPLDTHSP TNDVQPGRFS ASSLTASGQE SSNGTDRKTE LSELEDGSAA DWRRGVDPVS SRNAIGGGGI GHQKRKPDIM LPLFARPGMY PDPHSPFAVS PIPGRGGVLN VPISPALSLT PTIFSYSPSP GLSPFTSSSC FSFNPEEMKH YLHSQACSVF NYHLSPRTFP RYPGLMVPPL QCQMHPEEST QFSIKLQPPP VGRKNRERVE SSEESAPVTT PTMASIPPRI KVEPASEKDP ESLRQSAREK EEHTQEEGTV PSRTIEEEKG TIFARPAAPP IWPSVPISTP SGEPLEVTED SEDRPGKEPS APEKKEDALM PPKLRLKRRW NDDPEARELS KSGKFLWNGS GPQGLATAAA DA |
| 分子量 | 57.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为基于重组人ETV3蛋白相关研究的虚拟参考文献示例(仅供参考，非真实文献)：
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1. **文献名称**：*"ETV3 Functions as a Transcriptional Repressor to Regulate IFN-Responsive Genes in Macrophages"*
**作者**：Smith A et al. (2015)
**摘要**：本研究利用重组人ETV3蛋白进行体外实验，发现ETV3通过抑制干扰素(IFN)信号通路相关基因的转录，负调控巨噬细胞的炎症反应。研究揭示了ETV3在先天免疫中的潜在调节作用。
2. **文献名称**：*"Epigenetic Silencing of ETV3 in Acute Myeloid Leukemia Promotes Cell Survival"*
**作者**：Zhang L et al. (2018)
**摘要**：通过表达重组人ETV3蛋白，作者证实其在白血病细胞中诱导细胞凋亡，并抑制PI3K/AKT信号通路活性，表明ETV3可能作为AML中的肿瘤抑制因子。
3. **文献名称**：*"ETV3L Interacts with Herpes Simplex Virus 1 to Restrict Viral Replication"*
**作者**：Brown K et al. (2020)
**摘要**：使用重组人ETV3蛋白处理HSV-1感染细胞后，病毒复制显著受限。机制研究表明，ETV3通过增强宿主抗病毒基因(如IFITs)的表达抑制病毒增殖。
4. **文献名称**：*"Structural and Functional Analysis of ETV3 in TLR4-Mediated Inflammatory Pathways"*
**作者**：Lee J et al. (2022)
**摘要**：重组人ETV3蛋白的晶体结构解析表明其通过结合Toll样受体(TLR)信号下游分子，抑制NF-κB激活，提示其在炎症性疾病治疗中的潜在应用。
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**注**：以上文献为模拟示例，实际研究中请通过PubMed、Web of Science等平台以关键词“ETV3 recombinant protein”或“ETV3 function”检索真实文献。如需具体文献指引，建议补充更多研究背景或关键词。
Recombinant human ETV3 protein is a engineered version of the ETS translocation variant 3 (ETV3), a transcription factor belonging to the ETS family characterized by a conserved ETS DNA-binding domain. Naturally, ETV3 regulates gene expression by binding to specific DNA sequences, primarily functioning as a transcriptional repressor. It plays critical roles in cellular processes such as differentiation, proliferation, and immune regulation. Notably, ETV3 interacts with the STAT signaling pathway, where it collaborates with STAT3 to modulate immune responses and suppress oncogenic transformation. Dysregulation of ETV3 has been implicated in cancers, immune disorders, and viral pathogenesis, underscoring its dual role as a tumor suppressor and immune modulator. The recombinant form, typically produced in mammalian expression systems, retains the functional domains of native ETV3 and is utilized in research to study transcriptional mechanisms, immune tolerance, and cancer biology. Its applications include elucidating ETV3-STAT interactions, developing targeted therapies for malignancies, and exploring strategies to modulate immune checkpoints. Recent studies also highlight its potential in mitigating excessive inflammation, making it a candidate for treating autoimmune diseases. The production of recombinant ETV3 enables precise biochemical characterization and high-throughput screening for drug discovery.
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