| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ETV4 |
| Uniprot No | P43268 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-207aa |
| 氨基酸序列 | MYLHTEGFSGPSPGDGAMGYGYEKPLRPFPDDVCVAPEKFEGDIKQEGVGAFREGPPYQRRGALQLWQFLVALLDDPTNAHFIAWTGRGMEFKLIEPEEVARLWGIQKNRPAMNYDKLSRSLRYYYEKGIMQKVAGERYVYKFVCEPEALFSLAFPDNQRPALKAEFDRPVSEEDTVPLSHLDESPAYLPELAGPAQPFGPKGGYSY |
| 分子量 | 48.51 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ETV4蛋白的3篇代表性文献的简要概述(文献名与作者为虚构示例，仅供格式参考)：
1. **文献名称**：*Structural insights into human ETV4 transcription factor-DNA interactions*
**作者**：Zhang L, et al.
**摘要**：通过X射线晶体学解析重组人ETV4蛋白的DNA结合域结构，揭示其与靶基因启动子结合的分子机制，阐明关键氨基酸残基在特异性识别中的作用。
2. **文献名称**：*ETV4 promotes tumor metastasis via regulation of matrix metalloproteinases*
**作者**：Wang Y, et al.
**摘要**：利用重组人ETV4蛋白体外功能实验，证明其通过上调MMP-2/9表达促进癌细胞侵袭，RNA干扰沉默ETV4可抑制裸鼠移植瘤转移。
3. **文献名称**：*Functional characterization of recombinant ETV4 in embryonic development*
**作者**：Kimura T, et al.
**摘要**：在斑马鱼模型中注射重组人ETV4蛋白，发现其异常表达导致胚胎体轴发育缺陷，表明ETV4在Wnt信号通路中具有保守调控功能。
4. **文献名称**：*High-throughput screening identifies small-molecule inhibitors of ETV4 transcriptional activity*
**作者**：Smith J, et al.
**摘要**：开发基于重组ETV4蛋白的荧光素酶报告基因系统，筛选出可阻断其转录活性的化合物，为靶向ETV4的癌症治疗提供潜在药物候选分子。
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**注**：以上文献为示例模板，实际文献需通过PubMed或Google Scholar以“ETV4 recombinant protein”“ETV4 structure/function”等关键词检索获取。
Recombinant human ETV4 protein is a genetically engineered version of the ETS translocation variant 4 (ETV4), a transcription factor belonging to the ETS family. ETV4. also known as PEA3 or E1A-F, plays critical roles in regulating gene expression by binding to DNA through its conserved ETS domain. It is involved in developmental processes, cell differentiation, and tumorigenesis. Dysregulation of ETV4 has been linked to cancer progression, particularly in promoting metastasis by activating pathways related to cell migration and invasion (e.g., in prostate, breast, and lung cancers).
The recombinant protein is typically produced in bacterial or mammalian expression systems, enabling researchers to study its molecular mechanisms in vitro or in vivo. It retains functional properties, including DNA-binding and transactivation capabilities, making it valuable for investigating interactions with target genes, signaling pathways (e.g., MAPK/ERK), and co-regulatory proteins. Studies using recombinant ETV4 have highlighted its role in epithelial-mesenchymal transition (EMT) and its potential as a therapeutic target. Additionally, it serves as a tool for developing inhibitors or antibodies aimed at disrupting oncogenic ETV4 activity in preclinical models. Research continues to explore its context-dependent functions in both normal physiology and disease.
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