| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | EVI2B |
| Uniprot No | P34910 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-448aa |
| 氨基酸序列 | MDPKYFILILFCGHLNNTFFSKTETITTEKQSQPTLFTSSMSQVLANSQNTTGNPLGQPTQFSDTFSGQSISPAKVTAGQPTPAVYTSSEKPEAHTSAGQPLAYNTKQPTPIANASSQQAVFTSARQLPSARTSTTQPPKSFVYTFTQQSSSVQIPSRKQITVHNPSTQPTSTVKNSPRSTPGFILDTTSNKQTPQKNNYNSIAAILIGVLLTSMLVAIIIIVLWKCLRKPVLNDQNWAGRSLFADGETPGICMDNIRENEISTKRTSIISLTPWKPSKSTLLADDLEIKLFESSENIEDSNNPKTEKIKDQVNGTSEDSADGSTVGTAVSSSDDADLPPPPPLLDLEGQESNQSDKPTMTIVSPLPNDSTSLPPSLDCLNQDCGDHKSEIIQSFPPLDSLNLPLPPVDFMKNQEDSNLEIQCQEFSIPPNSDQDLNESLPPPPAELL |
| 分子量 | 75.68 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是为您整理的重组人EVI2B蛋白相关参考文献的示例(注：因文献数据库访问限制，以下内容为示例性虚构条目，建议通过PubMed、Google Scholar等平台验证真实文献)：
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1. **文献名称**: *"Cloning and Functional Characterization of Human EVI2B as a Regulator of Myeloid Differentiation"*
**作者**: Smith JL, et al.
**摘要**: 本研究成功在大肠杆菌系统中表达并纯化重组人EVI2B蛋白，通过体外实验证明其通过调控Wnt/β-catenin通路抑制急性髓系白血病(AML)细胞的分化，提示其在白血病发生中的潜在作用。
2. **文献名称**: *"Structural Analysis of Recombinant EVI2B Reveals a Novel Protein Interaction Domain"*
**作者**: Tanaka K, et al.
**摘要**: 利用X射线晶体学解析重组人EVI2B的晶体结构，发现其N端包含一个未被报道的蛋白质相互作用结构域，可能介导与胞内信号分子如CTNNB1(β-catenin)的结合。
3. **文献名称**: *"EVI2B Overexpression in Hematopoietic Stem Cells Promotes Leukemogenesis in Mouse Models"*
**作者**: Garcia-Ruiz C, et al.
**摘要**: 通过重组人EVI2B蛋白的体外递送实验，验证其在造血干细胞中的异常表达可导致基因组不稳定性和白血病转化，强调其致癌机制与DNA修复通路失调相关。
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**建议**：
如需真实文献，可通过以下关键词搜索：
- **"Recombinant human EVI2B protein expression"**
- **"EVI2B leukemia signaling"**
- **"EVI2B structure-function analysis"**
推荐数据库：NCBI PubMed (https://pubmed.ncbi.nlm.nih.gov)。
EVI2B (ectopic viral integration site 2 homolog B) is a human gene located on chromosome 17q21. originally identified due to its proximity to common retroviral integration sites in murine myeloid leukemia models. The encoded EVI2B protein is a transmembrane glycoprotein primarily expressed in hematopoietic cells, particularly myeloid lineages. Structurally, it contains an extracellular immunoglobulin-like domain, a single transmembrane region, and a cytoplasmic tail with potential signaling motifs. Alternative splicing generates multiple isoforms, though their functional distinctions remain under investigation.
Recombinant human EVI2B protein is typically produced using expression systems like mammalian cells (e.g., HEK293 or CHO) or *E. coli* (for non-glycosylated forms), followed by affinity chromatography purification. Its recombinant form enables studies on its role in cellular processes, including hematopoiesis, immune regulation, and leukemia pathogenesis. Aberrant EVI2B expression has been observed in acute myeloid leukemia (AML), where it may influence cell proliferation and survival pathways. Recent research also explores its potential as a tumor-associated antigen in immunotherapy and interactions with components of the tumor microenvironment. However, the exact molecular mechanisms and physiological ligands of EVI2B require further elucidation. Its recombinant version serves as a critical tool for functional assays, antibody development, and structural analyses aimed at understanding its dual roles in normal immunity and oncogenesis.
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