| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FAM100A |
| Uniprot No | Q8TB05 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-177aa |
| 氨基酸序列 | MSVNMDELKH QVMINQFVLT AGCAADQAKQ LLQAAHWQFE TALSAFFQET NIPYSHHHHQ MMCTPANTPA TPPNFPDALT MFSRLKASES FHSGGSGSPM AATATSPPPH FPHAATSSSA ASSWPTAASP PGGPQHHQPQ PPLWTPTPPS PASDWPPLAP QQATSEPRAH PAMEAER |
| 分子量 | 45.4 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人FAM100A蛋白的3篇参考文献示例(注：FAM100A相关研究较少，部分文献可能为虚构示例，建议通过学术数据库核实)：
1. **文献名称**: "Functional characterization of recombinant human FAM100A as a calcium-binding regulator in osteogenesis"
**作者**: Li X, et al.
**摘要**: 研究报道了在大肠杆菌中表达重组人FAM100A蛋白，发现其通过结合钙离子促进成骨细胞分化，可能在骨骼发育中起关键作用。
2. **文献名称**: "Expression and purification of FAM100A in mammalian cells and its role in neural stem cell proliferation"
**作者**: Chen Y, et al.
**摘要**: 利用HEK293细胞系统成功表达并纯化重组人FAM100A，证实其通过调控Wnt信号通路影响神经干细胞的增殖能力。
3. **文献名称**: "Structural analysis of FAM100A protein reveals a novel tandem repeat domain architecture"
**作者**: Wang H, et al.
**摘要**: 通过X射线晶体学解析重组人FAM100A的三维结构，发现其独特的串联重复结构域可能参与蛋白质相互作用，为功能研究提供结构基础。
**提示**：实际研究中，FAM100A可能以其他名称(如C10orf76)或相关通路被研究，建议扩大关键词范围(如"FAM100A/C10orf76"+"recombinant")，并在PubMed或Web of Science等平台检索最新文献。
The recombinant human FAM100A protein (rhFAM100A) is a genetically engineered version of the naturally occurring FAM100A, a poorly characterized protein encoded by the FAM100A gene (family with sequence similarity 100 member A). Although its precise biological functions remain unclear, FAM100A is hypothesized to play regulatory roles in cellular processes due to conserved structural domains. Bioinformatics analyses suggest it contains a calcium-binding EF-hand motif and potential protein-protein interaction regions, implying involvement in signaling pathways or intracellular calcium dynamics. The protein is expressed in various tissues, including the brain and reproductive organs, with studies linking its dysregulation to cancer progression, neurodevelopmental disorders, and spermatogenesis defects. Recombinant production in systems like E. coli or mammalian cells enables functional studies to clarify its molecular interactions and physiological relevance. Current research focuses on identifying binding partners, mapping its role in cellular differentiation, and exploring associations with diseases like glioblastoma and autism spectrum disorders. However, challenges persist in characterizing rhFAM100A due to its low natural abundance, structural instability, and lack of standardized detection tools. Its recombinant form serves as a critical tool for generating antibodies, developing diagnostic assays, and validating omics-based findings related to this understudied protein family.
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