| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FANCM |
| Uniprot No | Q8IYD8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-669aa |
| 氨基酸序列 | MSGRQRTLFQTWGSSISRSSGTPGCSSGTERPQSPGSSKAPLPAAAEAQLESDDDVLLVAAYEAERQLCLENGGFCTSAGALWIYPTNCPVRDYQLHISRAALFCNTLVCLPTGLGKTFIAAVVMYNFYRWFPSGKVVFMAPTKPLVTQQIEACYQVMGIPQSHMAEMTGSTQASTRKEIWCSKRVLFLTPQVMVNDLSRGACPAAEIKCLVIDEAHKALGNYAYCQVVRELVKYTNHFRILALSATPGSDIKAVQQVITNLLIGQIELRSEDSPDILTYSHERKVEKLIVPLGEELAAIQKTYIQILESFARSLIQRNVLMRRDIPNLTKYQIILARDQFRKNPSPNIVGIQQGIIEGEFAICISLYHGYELLQQMGMRSLYFFLCGIMDGTKGMTRSKNELGRNEDFMKLYNHLECMFARTRSTSANGISAIQQGDKNKKFVYSHPKLKKLEEVVIEHFKSWNAENTTEKKRDETRVMIFSSFRDSVQEIAEMLSQHQPIIRVMTFVGHASGKSTKGFTQKEQLEVVKQFRDGGYNTLVSTCVGEEGLDIGEVDLIICFDSQKSPIRLVQRMGRTGRKRQGRIVIILSEGREERIYNQSQSNKRSIYKAISSNRQVLHFYQRSPRMVPDGINPKLHKMFITHGVYEPEKPSRNLQRKSSIFSYRDGK |
| 分子量 | 102 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3-4条关于重组人FANCM蛋白的参考文献示例(注：文献为虚构示例，仅供格式参考)：
1. **"Structural Analysis of Recombinant Human FANCM Helicase Domain" by A. Lorenzo et al.**
摘要：通过X射线晶体学解析了重组人FANCM蛋白的螺旋酶结构域三维结构，揭示了其结合DNA并催化双链分离的分子机制。
2. **"FANCM Promotes Replication Forks Recovery by Counteracting Replication Stress" by J. Huang et al.**
摘要：研究表明，重组人FANCM蛋白通过调控复制叉的稳定性，促进DNA损伤后复制叉的重启，并协同其他FA蛋白维持基因组稳定性。
3. **"Biochemical Characterization of Recombinant Human FANCM ATPase Activity" by Q. Wen et al.**
摘要：通过体外表达纯化重组人FANCM蛋白，证实其具有ATP依赖性核酸解旋酶活性，并依赖完整的Walker B结构域实现功能。
4. **"FANCM Interaction with BLM Helicase in DNA Repair Pathways" by H. Kim et al.**
摘要：利用重组人FANCM蛋白与BLM蛋白进行共纯化实验，发现两者在体外形成复合物，协同参与同源重组修复中的DNA链间交联修复。
(提示：如需真实文献，请通过PubMed或Google Scholar检索关键词"Recombinant human FANCM"。)
Recombinant human FANCM protein is a genetically engineered version of the FANCM (Fanconi anemia complementation group M) protein, a critical DNA repair factor in the Fanconi anemia (FA) pathway. FANCM plays a central role in maintaining genome stability by resolving DNA interstrand crosslinks (ICLs) and replication stress. It functions as an ATP-dependent DNA translocase and serves as a scaffold for the FA core complex, facilitating the recruitment of downstream repair proteins. FANCM also stabilizes stalled replication forks and promotes homologous recombination repair. Defects in FANCM are linked to Fanconi anemia-like disorders, cancer predisposition, and infertility. Recombinant FANCM is typically produced using heterologous expression systems (e.g., Escherichia coli or mammalian cell lines) and purified for functional studies. Researchers use it to dissect molecular mechanisms of ICL repair, replication fork dynamics, and interactions with partner proteins like MHF1/MHF2. Its biochemical characterization has advanced understanding of how FA proteins cooperate in genome surveillance. Recent studies explore its potential as a therapeutic target for cancers with DNA repair deficiencies. In clinical research, recombinant FANCM aids in developing assays for diagnosing FA subtypes and evaluating chemotherapeutic responses in preclinical models.
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