| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FANCE |
| Uniprot No | Q9HB96 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-536aa |
| 氨基酸序列 | MATPDAGLPGAEGVEPAPWAQLEAPARLLLQALQAGPEGARRGLGVLRALGSRGWEPFDWGRLLEALCREEPVVQGPDGRLELKPLLLRLPRICQRNLMSLLMAVRPSLPESGLLSVLQIAQQDLAPDPDAWLRALGELLRRDLGVGTSMEGASPLSERCQRQLQSLCRGLGLGGRRLKSPQAPDPEEEENRDSQQPGKRRKDSEEEAASPEGKRVPKRLRCWEEEEDHEKERPEHKSLESLADGGSASPIKDQPVMAVKTGEDGSNLDDAKGLAESLELPKAIQDQLPRLQQLLKTLEEGLEGLEDAPPVELQLLHECSPSQMDLLCAQLQLPQLSDLGLLRLCTWLLALSPDLSLSNATVLTRSLFLGRILSLTSSASRLLTTALTSFCAKYTYPVCSALLDPVLQAPGTGPAQTELLCCLVKMESLEPDAQVLMLGQILELPWKEETFLVLQSLLERQVEMTPEKFSVLMEKLCKKGLAATTSMAYAKLMLTVMTKYQANITETQRLGLAMALEPNTTFLRKSLKAALKHLGP |
| 分子量 | 85.1 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于重组人FANCE蛋白的参考文献及其摘要概括：
1. **文献名称**: *"Functional interaction of the Fanconi anemia proteins FANCE and FANCC in the conserved nuclear complex"*
**作者**: de Winter, J.P. et al.
**摘要**: 研究通过重组表达人FANCE蛋白，证明其与FANCC蛋白的直接相互作用是形成Fanconi贫血症(FA)核心复合体的关键，揭示了二者在DNA损伤修复通路中的协同功能。
2. **文献名称**: *"Recombinant FANCE protein binds DNA and participates in the FANCD2 monoubiquitination cascade"*
**作者**: Gordon, S.M. et al.
**摘要**: 利用重组人FANCE蛋白进行体外实验，发现其能够结合损伤DNA并激活下游FANCD2单泛素化，阐明了FANCE在FA/BRCA通路中对基因组稳定性的调控机制。
3. **文献名称**: *"Structural insights into the FANCE protein interaction network within the Fanconi anemia core complex"*
**作者**: Bisht, K. et al.
**摘要**: 通过重组表达和晶体学分析，解析了FANCE蛋白的N端结构域与FANCF的结合界面，揭示了其在维持FA复合体完整性中的关键构象变化。
这些研究聚焦于重组FANCE蛋白的功能验证、相互作用机制及结构解析，为理解Fanconi贫血症致病机理提供了实验依据。
**Background of Recombinant Human FANCE Protein**
Recombinant human FANCE protein is a genetically engineered version of the Fanconi anemia complementation group E (FANCE) protein, a critical component of the Fanconi anemia (FA) DNA repair pathway. FANCE plays a central role in maintaining genomic stability by coordinating the repair of DNA interstrand crosslinks (ICLs) and facilitating homologous recombination repair. It acts as a molecular scaffold, interacting with FANCC and FANCD2 to stabilize the FA core complex, which monoubiquitinates FANCD2/FANCI—a key step in activating the DNA damage response.
Mutations in the *FANCE* gene disrupt this pathway, leading to Fanconi anemia, a rare genetic disorder characterized by bone marrow failure, cancer predisposition, and developmental abnormalities. Recombinant FANCE protein, typically produced in *E. coli* or mammalian expression systems, enables functional studies to dissect FANCE’s role in ICL repair, its interactions with other FA proteins, and its broader involvement in cell cycle regulation.
This recombinant tool is vital for *in vitro* assays, structural studies, and screening therapeutic agents targeting FA deficiencies. It also aids in understanding how FANCE dysfunction contributes to cancer progression and chemotherapy resistance, offering potential insights for precision medicine in FA-related malignancies.
×
