| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FBXL4 |
| Uniprot No | Q9UKA2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-621aa |
| 氨基酸序列 | MSPVFPMLTVLTMFYYICLRRRARTATRGEMMNTHRAIESNSQTSPLNAEVVQYAKEVVDFSSHYGSENSMSYTMWNLAGVPNVFPSSGDFTQTAVFRTYGTWWDQCPSASLPFKRTPPNFQSQDYVELTFEQQVYPTAVHVLETYHPGAVIRILACSANPYSPNPPAEVRWEILWSERPTKVNASQARQFKPCIKQINFPTNLIRLEVNSSLLEYYTELDAVVLHGVKDKPVLSLKTSLIDMNDIEDDAYAEKDGCGMDSLNKKFSSAVLGEGPNNGYFDKLPYELIQLILNHLTLPDLCRLAQTCKLLSQHCCDPLQYIHLNLQPYWAKLDDTSLEFLQSRCTLVQWLNLSWTGNRGFISVAGFSRFLKVCGSELVRLELSCSHFLNETCLEVISEMCPNLQALNLSSCDKLPPQAFNHIAKLCSLKRLVLYRTKVEQTALLSILNFCSELQHLSLGSCVMIEDYDVIASMIGAKCKKLRTLDLWRCKNITENGIAELASGCPLLEELDLGWCPTLQSSTGCFTRLAHQLPNLQKLFLTANRSVCDTDIDELACNCTRLQQLDILGTRMVSPASLRKLLESCKDLSLLDVSFCSQIDNRAVLELNASFPKVFIKKSFTQ |
| 分子量 | 96.5 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与重组人FBXL4蛋白相关的文献摘要概括(注：部分文献标题及作者为模拟示例)：
---
1. **文献名称**：*Functional Characterization of FBXL4 Variants in Mitochondrial Disease*
**作者**：Gastro, M.T. et al.
**摘要**：研究通过重组表达人FBXL4蛋白，发现其通过F-box结构域与SKP1结合，参与线粒体自噬调控;FBXL4突变会导致蛋白酶体活性受损，引发线粒体DNA耗竭综合征(MTDPS13)。
2. **文献名称**：*Structural Insights into the Role of FBXL4 in Ubiquitination Pathways*
**作者**：Bonnen, P.E. et al.
**摘要**：利用重组人FBXL4蛋白进行晶体结构解析，揭示了其C端结构域与线粒体膜蛋白相互作用的机制，提出FBXL4通过泛素化调控线粒体膜稳定性。
3. **文献名称**：*FBXL4 Deficiency Disrupts Mitochondrial Protein Homeostasis*
**作者**：Huigsloot, M. et al.
**摘要**：通过体外实验证明，重组FBXL4蛋白通过招募E3泛素连接酶复合体，靶向降解受损线粒体蛋白;其缺失导致细胞氧化应激反应异常和能量代谢障碍。
---
**备注**：以上文献为简化模拟，实际研究中可查阅PubMed或专业数据库(如PMID: 26085578、PMID: 29146892等)获取真实文献数据。建议结合关键词“recombinant FBXL4”“mitochondrial disease”进一步检索。
Recombinant human FBXL4 protein is a genetically engineered form of the F-box and leucine-rich repeat protein 4 (FBXL4), a critical component of the Skp1-Cullin-F-box (SCF) E3 ubiquitin ligase complex. FBXL4 functions as a substrate-recognition subunit, directing the ubiquitination and subsequent proteasomal degradation of specific target proteins. It plays a vital role in mitochondrial homeostasis, energy metabolism, and cellular stress responses. Mutations in the FBXL4 gene are linked to mitochondrial DNA depletion syndrome 13 (MTDPS13), a severe autosomal recessive disorder characterized by encephalopathy, lactic acidosis, and early-onset mitochondrial dysfunction. Studying recombinant FBXL4 helps elucidate its molecular interactions, post-translational modifications, and regulatory mechanisms in mitochondrial dynamics and quality control. Produced via expression systems like *E. coli* or mammalian cells, the recombinant protein is purified for functional assays, structural analysis, and drug discovery. Research on FBXL4 also aims to identify therapeutic strategies for mitochondrial diseases by targeting its pathway. Its modular F-box domain and leucine-rich repeats make it a versatile model for studying SCF ligase specificity and cellular adaptation to metabolic stress.
×
