| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FBXL21 |
| Uniprot No | Q9UKT6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-434aa |
| 氨基酸序列 | F box and leucine rich repeat protein 21; F box and leucine rich repeat protein 3 pseudogene; F box and leucine rich repeat protein 3B; F box protein Fbl3b; F box/LRR repeat protein 21; F box/LRR repeat protein 3B; F-box and leucine-rich repeat protein 21 |
| 分子量 | 49.1 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人FBXL21蛋白的假设性参考文献示例，供参考：
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1. **文献名称**：**"Structural Insights into Human FBXL21 Ubiquitination Complex by Cryo-EM"**
**作者**：Zhang Y. et al.
**摘要**：本研究通过冷冻电镜解析了重组人FBXL21蛋白与SCF泛素连接酶复合体的三维结构，揭示了其底物结合域的独特构象，并提出FBXL21通过特异性识别磷酸化修饰的靶蛋白，调控细胞周期进程。
2. **文献名称**：**"FBXL21 Regulates Circadian Clock via Proteasomal Degradation of CRY1"**
**作者**：Hirano A. et al.
**摘要**：作者发现重组人FBXL21蛋白通过泛素化降解核心时钟蛋白CRY1.影响哺乳动物昼夜节律。敲低FBXL21导致小鼠生物钟周期延长，表明其在节律调控中的关键作用。
3. **文献名称**：**"Recombinant FBXL21 Suppresses Tumor Growth by Targeting c-Myc for Degradation"**
**作者**：Wang L. et al.
**摘要**：研究证实，重组人FBXL21蛋白在多种癌细胞系中特异性结合并降解致癌蛋白c-Myc，抑制肿瘤增殖。体内实验显示FBXL21过表达可延缓异种移植瘤生长，提示其潜在治疗价值。
4. **文献名称**：**"FBXL21-Mediated Proteolysis Links Cellular Redox Stress to Mitochondrial Dynamics"**
**作者**：Kumar R. et al.
**摘要**：该研究揭示，重组人FBXL21蛋白在氧化应激条件下靶向线粒体融合蛋白MFN2进行泛素化降解，导致线粒体碎片化，从而影响细胞凋亡和能量代谢通路。
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**说明**：以上内容为假设性文献概括，实际研究中可能需结合具体数据库(如PubMed、Web of Science)检索。FBXL21相关研究多聚焦于其E3泛素连接酶活性及在代谢、癌症等领域的调控机制。
Recombinant human FBXL21 (F-box and leucine-rich repeat protein 21) is a genetically engineered protein belonging to the F-box family, which plays a critical role in the ubiquitin-proteasome system by targeting specific substrates for degradation. FBXL21 contains an F-box domain that facilitates interaction with Skp1. a core component of the SCF (Skp1-Cul1-F-box) E3 ubiquitin ligase complex. This complex mediates the transfer of ubiquitin to target proteins, marking them for proteasomal destruction. FBXL21 is implicated in diverse cellular processes, including circadian rhythm regulation, apoptosis, and metabolic homeostasis. Studies suggest its involvement in modulating the stability of key regulatory proteins, such as cryptochromes (CRY1/2), which are central to maintaining circadian clock function.
Recombinant FBXL21 is typically produced in bacterial or mammalian expression systems, enabling high-yield purification for biochemical and functional studies. Its recombinant form allows researchers to explore substrate specificity, enzymatic activity, and interaction partners in vitro. Aberrant expression or mutations in FBXL21 have been linked to circadian disorders and metabolic dysregulation, highlighting its potential therapeutic relevance. Current research focuses on elucidating its structural motifs, post-translational modifications, and regulatory networks to better understand its physiological and pathological roles. This protein remains a key target for studies on ubiquitination-mediated signaling and cellular homeostasis.
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