| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FBXL13 |
| Uniprot No | Q8NEE6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-735aa |
| 氨基酸序列 | MTPELMIKAC SFYTGHLVKT HFCTWRDIAR TNENVVLAEK MNRAVTCYNF RLQKSVFHHW HSYMEDQKEK LKNILLRIQQ IIYCHKLTII LTKWRNTARH KSKKKEDELI LKHELQLKKW KNRLILKRAA AEESNFPERS SSEVFLVDET LKCDISLLPE RAILQIFFYL SLKDVIICGQ VNHAWMLMTQ LNSLWNAIDF SSVKNVIPDK YIVSTLQRWR LNVLRLNFRG CLLRPKTFRS VSHCRNLQEL NVSDCPTFTD ESMRHISEGC PGVLCLNLSN TTITNRTMRL LPRHFHNLQN LSLAYCRRFT DKGLQYLNLG NGCHKLIYLD LSGCTQISVQ GFRYIANSCT GIMHLTINDM PTLTDNCVKA LVEKCSRITS LVFTGAPHIS DCTFRALSAC KLRKIRFEGN KRVTDASFKF IDKNYPNLSH IYMADCKGIT DSSLRSLSPL KQLTVLNLAN CVRIGDMGLK QFLDGPASMR IRELNLSNCV RLSDASVMKL SERCPNLNYL SLRNCEHLTA QGIGYIVNIF SLVSIDLSGT DISNEGLNVL SRHKKLKELS VSECYRITDD GIQAFCKSSL ILEHLDVSYC SQLSDMIIKA LAIYCINLTS LSIAGCPKIT DSAMEMLSAK CHYLHILDIS GCVLLTDQIL EDLQIGCKQL RILKMQYCTN ISKKAAQRMS SKVQQQEYNT NDPPRWFGYD REGNPVTELD NITSSKGALE LTVKKSTYSS EDQAA |
| 分子量 | 83.9 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人FBXL13蛋白的3条示例参考文献(内容为示例性虚构，供参考)：
---
1. **文献名称**：Structural and Functional Characterization of Recombinant Human FBXL13 Protein
**作者**：Zhang Y. et al.
**摘要**：本研究成功表达并纯化了重组人FBXL13蛋白，解析其晶体结构，揭示其F-box结构域与SCF(Skp1-Cul1-F-box)泛素连接酶复合体的结合模式，并验证其通过识别底物磷酸化位点参与细胞周期调控的功能。
2. **文献名称**：FBXL13 Regulates TGF-β Signaling by Targeting SMAD4 for Ubiquitination
**作者**：Li H. & Johnson R.
**摘要**：文章证明重组FBXL13蛋白通过与SMAD4相互作用介导其泛素化降解，从而负调控TGF-β信号通路，并发现FBXL13的缺失会导致肿瘤细胞迁移能力增强。
3. **文献名称**：High-Throughput Screening of FBXL13 Substrates Using Recombinant Protein Libraries
**作者**：Wang Q. et al.
**摘要**：通过构建重组FBXL13蛋白与人类蛋白质组文库的体外筛选体系，鉴定了多个潜在底物，包括代谢相关酶PKM2.提示FBXL13可能在糖酵解通路中发挥调控作用。
---
注：以上内容为模拟生成，实际研究中需查询真实数据库(如PubMed)获取文献。
FBXL13 is a member of the F-box and leucine-rich repeat (FBXL) protein family, characterized by an N-terminal F-box motif that facilitates interactions with SKP1 and the Cullin scaffold to form the SCF (SKP1-CUL1-F-box) ubiquitin ligase complex. As part of the ubiquitin-proteasome system, this complex targets specific proteins for degradation, a critical mechanism in regulating cellular processes such as cell cycle progression, signal transduction, and apoptosis. The FBXL13 protein also contains C-terminal leucine-rich repeats (LRRs), which are thought to mediate substrate recognition through protein-protein interactions.
Recombinant human FBXL13 protein is engineered using expression systems like *E. coli* or mammalian cells, enabling biochemical and functional studies. Its production allows researchers to investigate substrate specificity, enzymatic activity, and regulatory roles in cellular pathways. Although the precise biological functions of FBXL13 remain less characterized compared to other F-box proteins, emerging evidence suggests potential involvement in cancer, neurodevelopmental disorders, and immune responses. Dysregulation of SCF ligases, including those containing FBXL13. has been linked to pathological conditions due to altered protein turnover.
Current research focuses on identifying FBXL13-associated substrates and clarifying its mechanistic contributions to disease. Recombinant FBXL13 serves as a vital tool for *in vitro* assays, structural analysis, and drug discovery efforts targeting ubiquitination pathways. Further exploration may uncover therapeutic strategies for diseases driven by ubiquitin-proteasome system dysfunction.
×
