| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FBXL10 |
| Uniprot No | Q8NHM5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 457-555aa |
| 氨基酸序列 | LGKKPKAPALRFLKRTLSNESEESVKSTTLAVDYPKTPTGSPATEVSAKWTHLTEFELKGLKALVEKLESLPENKKCVPEGIEDPQALLEGVKNVLKEH |
| 分子量 | 36.63 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人FBXL10蛋白的3篇参考文献示例(文献信息为虚构，仅供格式参考)：
1. **文献名称**：*Structural insights into the substrate recognition of FBXL10 ubiquitin ligase*
**作者**：Zhang Y, et al.
**摘要**：研究解析了重组人FBXL10蛋白的晶体结构，揭示了其F-box和C端结构域在特异性识别靶标蛋白(如组蛋白甲基转移酶)中的作用机制，为泛素化调控提供新见解。
2. **文献名称**：*FBXL10-mediated histone H2A ubiquitination regulates gene silencing and cancer progression*
**作者**：Li H, et al.
**摘要**：通过体外重组实验证明，FBXL10作为SCF泛素连接酶复合物的底物识别亚基，催化H2A泛素化，参与染色质重塑和癌症相关基因的表观遗传沉默。
3. **文献名称**：*Functional characterization of recombinant FBXL10 in DNA damage response*
**作者**：Wang X, et al.
**摘要**：利用重组表达的FBXL10蛋白揭示其通过调控p53泛素化水平影响DNA损伤修复通路，表明其在细胞周期检查点和肿瘤抑制中的潜在功能。
(注：以上文献为模拟示例，实际引用需根据具体研究检索PubMed或Sci-Hub等数据库。)
FBXL10 (F-box and leucine-rich repeat protein 10), also known as KDM2B or JHDM1B, is a member of the F-box protein family that plays a critical role in epigenetic regulation and ubiquitin-mediated proteolysis. As a component of the SCF (SKP1-CUL1-F-box) E3 ubiquitin ligase complex, FBXL10 targets specific substrates for degradation via the ubiquitin-proteasome system. Notably, it contains a JmjC domain, enabling histone demethylase activity that specifically removes repressive histone H3K36me2/me1 marks, linking chromatin remodeling to transcriptional regulation. FBXL10 is involved in diverse biological processes, including stem cell maintenance, DNA damage response, cellular senescence, and tumor suppression or promotion, depending on context. Dysregulation of FBXL10 has been implicated in cancers, developmental disorders, and metabolic diseases. Recombinant human FBXL10 protein, produced through bacterial or mammalian expression systems, serves as a vital tool for in vitro studies to dissect its enzymatic activities, substrate interactions, and regulatory mechanisms. Researchers utilize purified FBXL10 to investigate its dual functions in chromatin modification (via demethylase assays) and protein ubiquitination (via reconstituted ligase assays), aiming to uncover therapeutic targets for related pathologies. Its structural domains, including the F-box, LRR, and JmjC motifs, are often studied for domain-specific functional characterization.
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