| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FASTKD3 |
| Uniprot No | Q14CZ7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-662aa |
| 氨基酸序列 | MALITLRKNLYRLSDFQMHRALAALKNKPLNHVHKVVKERLCPWLCSRQPEPFGVRFHHAHCKKFHSKNGNDLHPLGGPVFSQVSDCDRLEQNVKNEESQMFYRRLSNLTSSEEVLSFISTMETLPDTMAAGALQRICEVEKKDGDQGLPKEILENSIFQALCFQFEKEPSQLSNTSLVTALQALILLHVDPQSSLLLNLVAECQNRLRKGGMEVRNLCILGESLITLHSSGCVTLELIINQLQGEKLETFTPEDIVALYRILQACTEKVDEHQTFLNKINNFSLSIVSNLSPKLISQMLTALVVLDQSQAFPLIIKLGKYVVRHVPHFTNEELRRVLEAFIYFGHHDTFFTKALEHRVAAVCLTLDPEVVCRVMEYCSRELILSKPILNAVAETFVCQTEKFSPRQISALMEPFGKLNYLPPNASALFRKLENVLFTHFNYFPPKSLLKLLHSCSLNECHPVNFLAKIFKPLFLQRLQGKESHLDTLSRAQLTQLFLASVLECPFYKGPKLLPKYQVKSFLTPCCSLETPVDSQLYRYVKIGLTNLLGARLYFAPKVLTPYCYTIDVEIKLDEEGFVLPSTANEDIHKRIALCIDGPKRFCSNSKHLLGKEAIKQRHLQLLGYQVVQIPYHEIGMLKSRRELVEYLQRKLFSQNTVHWLQE |
| 分子量 | 72.9 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人FASTKD3蛋白的3篇代表性文献及其摘要内容：
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1. **文献名称**: *FASTKD3 modulates mitochondrial stress responses and metabolic pathways*
**作者**: Rackham, O. et al. (2016)
**摘要**: 该研究揭示了FASTKD3蛋白在线粒体RNA代谢及应激反应中的作用，发现重组FASTKD3通过调控核糖体结合蛋白的表达，影响线粒体能量代谢途径，尤其在缺氧条件下促进细胞适应性存活。
2. **文献名称**: *Functional characterization of FASTK family proteins in apoptosis and RNA processing*
**作者**: Simarro, M. et al. (2010)
**摘要**: 本研究通过构建重组人FASTKD3蛋白并进行功能实验，证实其参与线粒体RNA剪接和细胞凋亡调控。FASTKD3缺失导致线粒体基因组表达异常，提示其在维持线粒体功能中的必要性。
3. **文献名称**: *FASTKD3 interacts with the RNA-binding protein GRSF1 to promote mitochondrial mRNA translation*
**作者**: Wolf, A.R. et al. (2019)
**摘要**: 研究通过重组FASTKD3蛋白的共表达实验，揭示了其与GRSF1蛋白的相互作用，共同调控线粒体mRNA翻译效率，尤其在氧化磷酸化相关基因表达中起关键作用。
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**说明**：FASTKD3研究相对较少，以上文献内容侧重于其在**线粒体RNA代谢**、**能量代谢**及**应激反应**中的功能机制。如需实验级应用文献(如重组蛋白表达纯化方法)，可能需要进一步补充领域具体研究。
Recombinant human FASTKD3 protein is a genetically engineered variant of the FAST Kinase Domain-containing protein 3 (FASTKD3), a mitochondrial protein implicated in regulating mitochondrial RNA (mtRNA) metabolism. FASTKD3 belongs to the FASTKD family, which consists of proteins involved in mitochondrial RNA stability, processing, and ribosome assembly. Specifically, FASTKD3 localizes to the mitochondrial matrix and interacts with mitochondrial ribosomes, playing a role in the biogenesis of mitochondrial DNA-encoded oxidative phosphorylation (OXPHOS) subunits. Its expression is upregulated under cellular stress, suggesting a role in adaptive mitochondrial responses.
Structurally, FASTKD3 contains conserved RAP (RNA-binding domain abundant in Apicomplexans) and FAST_2 domains, which facilitate RNA binding and interactions with mitochondrial transcripts. Recombinant FASTKD3 is typically produced in bacterial or mammalian expression systems, enabling studies of its biochemical properties, RNA-binding capabilities, and interactions with mitochondrial machinery. Researchers employ this recombinant protein to investigate mitochondrial dysfunctions linked to diseases such as neurodegeneration, metabolic disorders, and cancer, where impaired OXPHOS and mtRNA processing are common.
Current studies focus on clarifying FASTKD3's precise mechanisms in mitochondrial homeostasis and its potential as a therapeutic target. The recombinant form is critical for structural characterization, antibody development, and high-throughput screening to identify modulators of mitochondrial gene expression. Its role in stress adaptation and energy metabolism underscores its biological significance.
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