| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FARP2 |
| Uniprot No | O94887 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 44-324aa |
| 氨基酸序列 | LHLRVKLLDNTMEIFDIEPKCDGQVLLTQVWKRLNLVECDYFGMEFQNTQSYWIWLEPMKPIIRQIRRPKNVVLRLAVKFFPPDPGQLQEEYTRYLFALQLKRDLLEERLTCADTTAALLTSHLLQSEIGDYDETLDREHLKVNEYLPGQQHCLEKILEFHQKHVGQTPAESDFQVLEIARKLEMYGIRFHMASDREGTKIQLAVSHMGVLVFQGTTKINTFNWSKVRKLSFKRKRFLIKLHPEVHGPYQDTLEFLLGSRDECKNFWKICVEYHTFFRLLD |
| 分子量 | 40.3 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人FARP2蛋白的模拟参考文献示例(请注意这些为构造内容，非真实文献)：
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1. **文献名称**: *Recombinant human FARP2 purification and its role in Wnt/PCP signaling*
**作者**: Zhang L, et al.
**摘要**: 本研究利用大肠杆菌系统成功表达并纯化了重组人FARP2蛋白，通过体外实验验证其作为RhoGEF活性调节因子的功能，并发现其参与调控Wnt/PCP信号通路中细胞极性的建立。
2. **文献名称**: *Structural and functional analysis of FARP2’s PH domain in neurite outgrowth*
**作者**: Tanaka K, et al.
**摘要**: 通过HEK293细胞表达重组FARP2蛋白，结合晶体结构分析和神经元培养实验，揭示了FARP2的PH结构域与膜脂质互作机制，及其在轴突生长中的关键作用。
3. **文献名称**: *FARP2 interacts with GPR126 to mediate Schwann cell development*
**作者**: Melfi S, et al.
**摘要**: 研究利用重组FARP2蛋白进行体外结合实验，证实其与G蛋白偶联受体GPR126的直接相互作用，并阐明该复合物在施万细胞分化及髓鞘形成中的调控网络。
4. **文献名称**: *Development of a FARP2 activity assay using recombinant protein*
**作者**: Gupta R, et al.
**摘要**: 开发基于重组FARP2蛋白的高通量GEF活性检测方法，筛选小分子抑制剂并评估其对肿瘤细胞迁移的抑制作用，为靶向治疗提供新思路。
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**备注**：上述文献为示例，实际研究中请通过PubMed或Web of Science等平台查找真实发表文献。
**Background of Recombinant Human FARP2 Protein**
FARP2 (FERM, ARH/RhoGEF, and Pleckstrin Domain Protein 2) is a multidomain signaling protein that belongs to the Rho guanine nucleotide exchange factor (GEF) family. It plays a critical role in regulating cytoskeletal dynamics, cell migration, and intracellular signaling by activating Rho GTPases, particularly Rac1 and Cdc42. Structurally, FARP2 contains a FERM domain (involved in membrane association and protein interactions), a DH/PH tandem domain (responsible for GEF activity), and a C-terminal pleckstrin homology (PH) domain that binds phospholipids.
Recombinant human FARP2 protein is produced using heterologous expression systems (e.g., bacterial or mammalian cells) to enable functional and structural studies. Its production facilitates investigations into neuronal development, synaptic plasticity, and cancer metastasis, as aberrant FARP2 activity is linked to neurological disorders and tumor invasiveness. Researchers employ recombinant FARP2 to dissect its role in signal transduction pathways, screen for therapeutic agents, and study its interactions with downstream effectors. Its biochemical characterization aids in understanding disease mechanisms and identifying potential targets for drug development.
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