| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FBRS |
| Uniprot No | Q9HAH7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-460aa |
| 氨基酸序列 | MFEKYPGKMEGLFRHNPYTAFPPAVPGLPPGLPPAVSFGSLQGAFQPKSTNPELPPRLGPVPSGLSQKGTQIPDHFRPPLRKPGKWCAMHVRVAYMILRHQEKMKGDSHKLDFRNDLLPCLPGPYGALPPGQELSHPASLFTATGAVHAAANPFTAAPGAHGPFLSPSTHIDPFGRPTSFASLAALSNGAFGGLGSPTFNSGAVFAQKESPGAPPAFASPPDPWGRLHRSPLTFPAWVRPPEAARTPGSDKERPVERREPSITKEEKDRDLPFSRPQLRVSPATPKARAGEEGPRPTKESVRVKEERKEEAAAAAAAAAAAAAAAAAAATGPQGLHLLFERPRPPPFLGPSPPDRCAGFLEPTWLAAPPRLARPPRFYEAGEELTGPGAVAAARLYGLEPAHPLLYSRLAPPPPPAAAPGTPHLLSKTPPGALLGAPPPLVPAPRPSSPPRGPGPARADR |
| 分子量 | 48.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是基于重组蛋白研究领域常见方向构造的3条示例参考文献(虚构，仅供参考)：
---
1. **文献名称**: "Cloning and Functional Characterization of Recombinant Human FBRS Protein in Bacterial Expression Systems"
**作者**: Zhang Y. et al.
**摘要**: 该研究成功克隆了人源FBRS基因，并利用大肠杆菌表达系统实现了重组蛋白的高效表达与纯化。通过体外实验证实，重组FBRS蛋白具有结合DNA的活性，提示其可能在染色质调控中发挥作用。
---
2. **文献名称**: "Structural Insights into the FBRS Protein: Crystallographic Analysis of Recombinant Human FBRS"
**作者**: Li X. et al.
**摘要**: 本研究解析了重组人FBRS蛋白的晶体结构，揭示了其独特的C端结构域对维持蛋白稳定性至关重要。结构比对表明，FBRS可能通过特定的结构基序参与蛋白质相互作用。
---
3. **文献名称**: "FBRS Knockdown Impairs Cell Cycle Progression: Mechanistic Studies Using Recombinant FBRS Protein"
**作者**: Wang H. et al.
**摘要**: 通过结合RNA干扰技术与体外添加重组FBRS蛋白的功能回补实验，作者发现FBRS通过调控CDK2的磷酸化水平影响细胞周期G1/S期转换，提示其潜在肿瘤治疗靶点价值。
---
**备注**：以上文献为模拟示例，实际研究中需核实"FBRS"是否为标准命名。建议通过PubMed或Google Scholar以“recombinant human FBRS protein”或“FBRS gene function”等关键词检索最新文献。若涉及拼写疑义，可确认目标蛋白的全称(如Fibrosheathin-Binding RS Domain protein等)。
Recombinant human FBRS (Fibrosin-1) protein is a biologically active form of the FBRS protein, encoded by the *FBRS* gene located on human chromosome 16. FBRS is a conserved mitotic regulator involved in spindle assembly and chromosome segregation during cell division. It plays a critical role in maintaining genomic stability by facilitating proper microtubule-kinetochore interactions and ensuring accurate mitotic progression. Structurally, FBRS contains multiple coiled-coil domains and a conserved C-terminal region, enabling interactions with other spindle-associated proteins like Aurora B kinase and components of the chromosomal passenger complex. Dysregulation of FBRS has been implicated in mitotic errors, aneuploidy, and potential links to cancer or neurodevelopmental disorders. Recombinant FBRS is typically produced in *E. coli* or mammalian expression systems, followed by purification via affinity chromatography. Its applications include functional studies of spindle dynamics, biochemical assays to map protein interaction networks, and high-throughput screens to identify compounds targeting mitotic pathways. Researchers also use it to explore FBRS's role in diseases or as a potential diagnostic biomarker. The recombinant form offers a standardized tool for dissecting molecular mechanisms underlying cell division and developing therapeutic strategies for mitotic dysfunction-related pathologies.
×
