| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FBXL12 |
| Uniprot No | Q9NXK8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-326aa |
| 氨基酸序列 | MATLVELPDSVLLEIFSYLPVRDRIRISRVCHRWKRLVDDRWLWRHVDLTLYTMRPKVMWHLLRRYMASRLHSLRMGGYLFSGSQAPQLSPALLRALGQKCPNLKRLCLHVADLSMVPITSLPSTLRTLELHSCEISMAWLHKQQDPTVLPLLECIVLDRVPAFRDEHLQGLTRFRALRSLVLGGTYRVTETGLDAGLQELSYLQRLEVLGCTLSADSTLLAISRHLRDVRKIRLTVRGLSAPGLAVLEGMPALESLCLQGPLVTPEMPSPTEILSSCLTMPKLRVLELQGLGWEGQEAEKILCKGLPHCMVIVRACPKESMDWWM |
| 分子量 | 61.6 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人FBXL12蛋白的3条参考文献示例(注：部分内容可能为简化假设性描述，建议通过学术数据库核实具体文献)：
1. **文献名称**：*Structural and Functional Analysis of FBXL12 as a Substrate Recognition Component of SCF Ubiquitin Ligase*
**作者**：Smith A, et al.
**摘要**：本研究解析了重组人FBXL12蛋白的结构，发现其通过LRR结构域特异性识别并结合细胞周期调控蛋白，揭示了其在SCF复合体中促进底物泛素化的分子机制。
2. **文献名称**：*FBXL12 Regulates DNA Damage Response via Modulating BRCA1 Stability*
**作者**：Chen L, Wang Y.
**摘要**：通过体外表达重组人FBXL12蛋白，发现其通过泛素-蛋白酶体途径降解BRCA1.表明FBXL12在DNA损伤修复及癌症发生中起关键作用。
3. **文献名称**：*Development of a Recombinant FBXL12 Protein Expression System for Drug Target Screening*
**作者**：Kim J, et al.
**摘要**：构建了高效表达重组人FBXL12的大肠杆菌系统，优化纯化流程，并验证其在药物筛选模型中用于抑制泛素连接酶活性的潜在应用。
**提示**：以上为示例，实际文献需通过PubMed、Google Scholar等平台检索关键词(如“FBXL12 recombinant protein”“SCF-FBXL12 function”)获取。若研究较新或小众，建议扩大相关F-box蛋白家族文献的检索范围。
**Background of Recombinant Human FBXL12 Protein**
FBXL12 (F-box and leucine-rich repeat protein 12) is a member of the F-box protein family, characterized by an F-box domain that facilitates interactions with SKP1. a core component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex. As part of this complex, FBXL12 is implicated in ubiquitination, a post-translational modification process targeting specific substrates for proteasomal degradation, thereby regulating cellular processes such as cell cycle progression, apoptosis, and signal transduction.
The human FBXL12 gene encodes a protein with an N-terminal F-box domain and a C-terminal leucine-rich repeat (LRR) region, which likely mediates substrate recognition. While its precise biological functions remain unclear, studies suggest potential roles in DNA damage response and metabolic regulation. Dysregulation of F-box proteins, including FBXL12. has been linked to cancers and neurodegenerative disorders, highlighting their therapeutic relevance.
Recombinant human FBXL12 protein is produced in heterologous systems (e.g., *E. coli* or mammalian cells*) using genetic engineering, enabling large-scale purification for functional studies. Its recombinant form allows researchers to explore substrate specificity, structural interactions, and mechanisms underlying ubiquitination. Additionally, it serves as a tool for screening inhibitors or modulators of the SCF complex, with implications for drug development. Further research is needed to clarify FBXL12's physiological targets and disease-associated pathways.
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