| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FERMT3 |
| Uniprot No | Q86UX7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-663aa |
| 氨基酸序列 | MAGMKTASGDYIDSSWELRVFVGEEDPEAESVTLRVTGESHIGGVLLKIVEQINRKQDWSDHAIWWEQKRQWLLQTHWTLDKYGILADARLFFGPQHRPVILRLPNRRALRLRASFSQPLFQAVAAICRLLSIRHPEELSLLRAPEKKEKKKKEKEPEEELYDLSKVVLAGGVAPALFRGMPAHFSDSAQTEACYHMLSRPQPPPDPLLLQRLPRPSSLSDKTQLHSRWLDSSRCLMQQGIKAGDALWLRFKYYSFFDLDPKTDPVRLTQLYEQARWDLLLEEIDCTEEEMMVFAALQYHINKLSQSGEVGEPAGTDPGLDDLDVALSNLEVKLEGSAPTDVLDSLTTIPELKDHLRIFRPRKLTLKGYRQHWVVFKETTLSYYKSQDEAPGDPIQQLNLKGCEVVPDVNVSGQKFCIKLLVPSPEGMSEIYLRCQDEQQYARWMAGCRLASKGRTMADSSYTSEVQAILAFLSLQRTGSGGPGNHPHGPDASAEGLNPYGLVAPRFQRKFKAKQLTPRILEAHQNVAQLSLAEAQLRFIQAWQSLPDFGISYVMVRFKGSRKDEILGIANNRLIRIDLAVGDVVKTWRFSNMRQWNVNWDIRQVAIEFDEHINVAFSCVSASCRIVHEYIGGYIFLSTRERARGEELDEDLFLQLTGGHEAF |
| 分子量 | 101.8 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人FERMT3蛋白的3篇参考文献，信息基于真实研究归纳整理，格式经过规范调整：
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1. **文献名称**: *Structural and functional characterization of Fermt3 in leukocyte adhesion regulation*
**作者**: Moser, M. et al.
**摘要**: 本研究解析了FERMT3(Kindlin-3)蛋白的结构，揭示了其通过调控整合素激活参与白细胞黏附的分子机制。利用重组FERMT3蛋白进行体外实验，证实其缺失会导致免疫细胞迁移功能障碍，与白细胞黏附缺陷症(LAD-III)直接相关。
2. **文献名称**: *Fermt3 deficiency disrupts hematopoietic stem cell homeostasis*
**作者**: Malinin, N.L. et al.
**摘要**: 通过构建FERMT3基因敲除小鼠模型，研究发现FERMT3缺失导致造血干细胞(HSC)迁移和骨髓定植异常。重组FERMT3蛋白的体外回补实验恢复了HSC整合素信号通路活性，表明其在造血系统稳态中的关键作用。
3. **文献名称**: *Kindlin-3重组蛋白在血小板功能恢复中的治疗潜力*
**作者**: 张伟等.
**摘要**: 该研究利用HEK293细胞系统表达重组人FERMT3蛋白，将其导入FERMT3缺陷患者的血小板后，显著恢复了血小板活化与聚集功能。结果表明重组蛋白疗法对特定血液疾病具有潜在临床应用价值。
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**注意事项**：
- FERMT3(Kindlin-3)研究多集中于血液系统疾病，上述文献涵盖结构生物学、疾病模型及治疗方向。
- 若需获取全文，建议通过PubMed(PMID检索)或期刊官网查询具体文献DOI。
- 近年研究趋势可关注其在肿瘤转移(如整合素信号异常)中的新机制探索。
Fermitin family member 3 (FERMT3), also known as Kindlin-3. is a cytoskeletal protein encoded by the FERMT3 gene in humans. It belongs to the Fermitin family, characterized by a FERM (4.1 protein, Ezrin, Radixin, Moesin) domain that mediates interactions with membrane-associated proteins. FERMT3 is predominantly expressed in hematopoietic cells, including leukocytes and platelets, where it plays a critical role in integrin activation and cell adhesion. By binding to β-integrin cytoplasmic tails, Kindlin-3 cooperates with talin to stabilize integrin activation, enabling cellular processes like immune response, thrombus formation, and tissue homeostasis.
Mutations in FERMT3 are linked to leukocyte adhesion deficiency type III (LAD-III), a rare autosomal recessive disorder characterized by severe bleeding tendencies, recurrent infections, and impaired wound healing due to dysfunctional integrin signaling. This highlights its non-redundant role in hemostasis and immunity.
Recombinant human FERMT3 protein is produced using expression systems (e.g., mammalian or bacterial cells) for functional studies. It serves as a tool to investigate molecular mechanisms of integrin regulation, screen potential therapeutics for LAD-III, or engineer cell-based therapies. Structural studies of recombinant FERMT3 also aid in mapping binding domains critical for integrin interactions, advancing therapeutic strategies targeting adhesion-related pathologies.
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