| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GAS2L3 |
| Uniprot No | Q86XJ1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-694aa |
| 氨基酸序列 | MQPAIQVWFGEDLPLSPRSPLTPRHGPGLANVCQYDEWIAVRHEATLLPMQEDLSIWLSGLLGIKVKAEKLLEELDNGVLLCQLIDVLQNMVKTCNSEESGNFPMRKVPCKKDAASGSFFARDNTANFLHWCRDIGVDETYLFESEGLVLHKDPRQVYLCLLEIGRIVSRYGVEPPVLVKLEKEIELEETLLNTSGPEDSISIPKSCCRHEELHEAVKHIAEDPPCSCSHRFSIEYLSEGRYRLGDKILFIRMLHGKHVMVRVGGGWDTLQGFLLKYDPCRILQFATLEQKILAFQKGVSNESVPDSPARTPQPPEMNPLSAVNMFQKQNSKPSVPVSIPKSKEKQGRPPGALVPASSLKGGNLGSMSVRSKLPNSPAASSHPKLKSSKGITKKPQAPSNNASSSLASLNPVGKNTSSPALPRTAPCISESPRKCISSPNTPKAKVIPAQNSADLPESTLLPNKCSGKTQPKYLKHNHISSRDNAVSHLAAHSNSSSKCPKLPKANIPVRPKPSFQSSAKMTKTSSKTIATGLGTQSQPSDGAPQAKPVPAQKLKSALNLNQPVSVSSVSPVKATQKSKDKNIVSATKKQPQNKSAFQKTGPSSLKSPGRTPLSIVSLPQSSTKTQTAPKSAQTVAKSQHSTKGPPRSGKTPASIRKPPSSVKDADSGDKKPTAKKKEDDDHYFVMTGSKKPRK |
| 分子量 | 101.6 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为假想的重组人GAS2L3蛋白相关参考文献示例(实际文献需通过学术数据库验证)：
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1. **"Recombinant human GAS2L3 protein modulates cytoskeletal dynamics in mitotic cells"**
*Authors: Zhang Y., Wang X., et al.*
**摘要**：研究报道了重组人GAS2L3蛋白的表达及纯化方法，发现其通过结合微管和微丝调控细胞分裂过程中的骨架重塑，影响纺锤体稳定性与胞质分裂。
2. **"Functional interaction of GAS2L3 with survivin in apoptosis regulation"**
*Authors: Müller R., Schmidt A., et al.*
**摘要**：发现GAS2L3通过结合凋亡抑制蛋白survivin调控 caspase 通路，重组蛋白实验表明其在DNA损伤后促进caspase-3活化，提示潜在促凋亡功能。
3. **"Structural basis of GAS2L3-mediated microtubule stabilization"**
*Authors: Chen L., Ito H., et al.*
**摘要**：通过X射线晶体学解析GAS2L3的微管结合结构域，揭示其通过钙离子依赖机制稳定微管，可能为肿瘤治疗提供新靶点。
4. **"GAS2L3 suppresses epithelial-mesenchymal transition in breast cancer cells"**
*Authors: Li J., Kim T., et al.*
**摘要**：体外实验显示重组GAS2L3过表达抑制TGF-β诱导的EMT，降低迁移侵袭能力，可能与抑制Wnt/β-catenin通路相关。
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**提示**：以上为模拟示例，建议通过PubMed、Web of Science或Google Scholar以“recombinant human GAS2L3”为关键词检索真实文献。如需具体文献协助，请提供更详细的研究背景。
Recombinant human GAS2L3 (Growth Arrest-Specific 2 Like 3) is a protein encoded by the GAS2L3 gene, belonging to the GAS2 family of microtubule-associated proteins. This family is characterized by roles in cytoskeletal regulation and cellular processes linked to growth arrest. GAS2L3 contains a conserved GAS2-related (GAR) domain and a calponin homology (CH) domain, enabling interactions with microtubules and actin filaments, thereby influencing cytoskeletal dynamics. It is implicated in regulating mitotic progression, particularly during cytokinesis, where it may coordinate cytoskeletal remodeling for proper cell division. Dysregulation of GAS2L3 has been associated with mitotic defects, genomic instability, and potential links to cancer progression. Recombinant GAS2L3 is typically produced in heterologous expression systems (e.g., mammalian or insect cells) using DNA cloning techniques to enable controlled overexpression, followed by purification via affinity chromatography. Its recombinant form facilitates in vitro studies to dissect molecular interactions, structural features, and functional mechanisms. Research on GAS2L3 contributes to understanding cell cycle regulation, mitotic fidelity, and pathological conditions involving cytoskeletal dysfunctions. It also serves as a tool for screening therapeutic agents targeting cell division abnormalities in cancer and developmental disorders.
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