| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GDDR |
| Uniprot No | Q86XP6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-184aa |
| 氨基酸序列 | MKILVAFLVVLTIFGIQSHGYEVFNIISPSNNGGNVQETVTIDNEKNTAIINIHAGSCSSTTIFDYKHGYIASRVLSRRACFILKMDHQNIPPLNNLQWYIYEKQALDNMFSSKYTWVKYNPLESLIKDVDWFLLGSPIEKLCKHIPLYKGEVVENTHNVGAGGCAKAGLLGILGISICADIHV |
| 分子量 | 46.9 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人GDDR蛋白的虚构参考文献示例(请注意，“GDDR”可能是拼写错误或非常规缩写，实际文献需核实名称准确性)：
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1. **文献名称**：High-Yield Production of Recombinant Human GDDR Protein Using a Mammalian Expression System
**作者**：Chen W, et al.
**摘要**：研究利用HEK293细胞系统高效表达并纯化重组人GDDR蛋白，通过质谱和免疫印迹验证其正确折叠及翻译后修饰，为后续功能研究提供高质量蛋白来源。
2. **文献名称**：Crystal Structure Analysis of GDDR Reveals a Unique Receptor-Ligand Binding Mechanism
**作者**：Thompson R, et al.
**摘要**：首次解析GDDR蛋白的晶体结构，发现其具有非典型的配体结合口袋，并通过分子对接实验预测了潜在小分子抑制剂，为靶向治疗提供结构依据。
3. **文献名称**：GDDR-Mediated Signaling Pathway in Inflammatory Response: Role of Recombinant Protein in Macrophage Activation
**作者**：Saito M, et al.
**摘要**：通过体外实验证明重组GDDR蛋白可激活巨噬细胞中NF-κB通路，加剧炎症因子释放，提示其在自身免疫疾病中的潜在作用。
4. **文献名称**：Recombinant GDDR as a Diagnostic Marker for Early-Stage Neurodegenerative Diseases
**作者**：Gupta P, et al.
**摘要**：检测到脑脊液中重组GDDR蛋白水平与阿尔茨海默病进展呈正相关，提出其作为神经退行性疾病早期诊断标志物的可能性。
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**备注**：若实际研究中无“GDDR”相关文献，建议核对名称是否正确(如是否为DDR1、GDNF受体等)，或结合具体研究领域调整关键词检索。
Recombinant human GDDR protein is a genetically engineered form of the Glial Cell Line-Derived Neurotrophic Factor (GDNF) receptor, typically produced using expression systems like bacteria, yeast, or mammalian cells. GDDR, part of the GDNF family receptor α (GFRα) group, plays a critical role in binding neurotrophic factors such as GDNF, neurturin, and artemin. These ligands activate RET receptor tyrosine kinase signaling, regulating cell survival, differentiation, and maintenance in the nervous system, particularly in dopaminergic, enteric, and sensory neurons.
The recombinant version is widely used to study neurodegenerative diseases (e.g., Parkinson’s), cancer, and metabolic disorders, as aberrant GDNF signaling is linked to tumorigenesis and neuronal degeneration. Its production enables controlled in vitro studies, drug screening, and therapeutic development. Structurally, it includes ligand-binding domains to facilitate interaction studies, aiding in elucidating molecular mechanisms or designing targeted therapies.
Challenges in its application include maintaining proper post-translational modifications and solubility, often addressed through codon optimization or fusion tags. Current research focuses on optimizing delivery systems and enhancing stability for clinical translation, such as neuroprotective treatments or anticancer strategies. Its versatility underscores its importance in both basic research and biopharmaceutical innovation.
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