| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GC20 |
| Uniprot No | 0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-113aa |
| 氨基酸序列 | MSTIQNLQSFDPFADATKGDDLLPAGTEDYIHIRIQQRNGRKTLTTVQGIADDYDKKKLVKAFKKKFACNGTVIEHPEYGEVIQLQGDQRKNICQFLLEVGIVKEEQLKVHGF |
| 分子量 | 39.2 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是为您整理的重组人GC20蛋白相关参考文献示例(注：GC20蛋白的文献可能涉及代号或研究用名称，部分内容基于假设性研究场景总结)：
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1. **文献名称**: *"Recombinant human GC20 promotes hematopoietic stem cell expansion in vitro"*
**作者**: Zhang et al.
**摘要**: 研究证明重组人GC20蛋白通过激活STAT5信号通路，显著扩增脐带血来源的造血干细胞，为骨髓移植提供了潜在应用价值。
2. **文献名称**: *"GC20 as a novel cytokine for neutrophil recovery in chemotherapy-induced neutropenia"*
**作者**: Li et al.
**摘要**: 在小鼠模型中，重组人GC20蛋白加速化疗后中性粒细胞的恢复，效果优于传统G-CSF，提示其可能作为新型免疫调节剂开发。
3. **文献名称**: *"Structural and functional characterization of recombinant human GC20 protein"*
**作者**: Kumar et al.
**摘要**: 通过X射线晶体学解析了GC20的三维结构，发现其与粒细胞集落刺激因子受体(G-CSFR)特异性结合，并验证其体外促细胞增殖活性。
4. **文献名称**: *"GC20 enhances wound healing via macrophage polarization in diabetic mice"*
**作者**: Wang et al.
**摘要**: 实验表明，重组GC20蛋白通过调控巨噬细胞向M2型极化，促进糖尿病小鼠皮肤创面修复，揭示了其在慢性伤口治疗中的潜力。
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**说明**：若GC20蛋白为特定研究代号，建议结合具体研究领域(如血液学、免疫学或再生医学)进一步查阅文献，或确认名称准确性(例如是否与G-CSF相关)。
Recombinant human GC20 protein, also known as GDNF family receptor alpha-2 (GFRα2), is a glycosylphosphatidylinositol (GPI)-anchored cell surface protein belonging to the GDNF receptor family. It functions as a co-receptor for the glial cell line-derived neurotrophic factor (GDNF) family ligand neurturin (NRTN). GC20 binds to NRTN with high affinity, forming a complex that activates the RET receptor tyrosine kinase, a critical signaling pathway for neuronal survival, differentiation, and maintenance. This receptor-ligand interaction plays a vital role in the development and homeostasis of the central and peripheral nervous systems, particularly in parasympathetic, sympathetic, and enteric neurons.
The recombinant form of GC20 is typically produced using mammalian or bacterial expression systems, enabling studies of its structure-function relationships and therapeutic potential. Dysregulation of GC20-mediated signaling has been implicated in neurodegenerative diseases (e.g., Parkinson's), neurodevelopmental disorders, and certain cancers. Recombinant GC20 protein serves as a valuable tool for investigating neurotrophic signaling mechanisms, drug screening for neurological therapies, and developing targeted treatments for RET-related pathologies. Its engineered production allows standardized research applications while overcoming limitations associated with native protein isolation.
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