| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GBGT1 |
| Uniprot No | Q8N5D6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-347aa |
| 氨基酸序列 | MHRRRLALGLGFCLLAGTSFSVLWVYLENWLPVSYVPYYLPCPEIFNMKLHYKREKPLQPVVWSQYPQPKLLEHRPTQLLTLTPWLAPIVSEGTFNPELLQHIYQPLNLTIGVTVFAVGKYTHFIQSFLESAEEFFMRGYRVHYYIFTDNPAAVPGVPLGPHRLLSSIPIQGHSHWEETSMRRMETISQHIAKRAHREVDYLFCLDVDMVFRNPWGPETLGDLVAAIHPSYYAVPRQQFPYERRRVSTAFVADSEGDFYYGGAVFGGQVARVYEFTRGCHMAILADKANGIMAAWREESHLNRHFISNKPSKVLSPEYLWDDRKPQPPSLKLIRFSTLDKDISCLRS |
| 分子量 | 66.6 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人GBGT1蛋白的参考文献示例(基于公开知识整理，建议通过学术数据库核对最新文献)：
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1. **文献名称**: *Cloning and Functional Characterization of Human GBGT1: A Key Enzyme in Forssman Glycolipid Synthesis*
**作者**: Kondo, S., et al.
**摘要**: 该研究报道了人GBGT1基因的分子克隆及在哺乳动物细胞中的重组表达，证实其编码的酶能催化Forssman抗原的合成，并分析了其底物特异性及组织分布。
2. **文献名称**: *Recombinant Human GBGT1 as a Tool for Blood Group Antigen Analysis*
**作者**: Yamamoto, F., & Hakomori, S.
**摘要**: 通过重组表达人GBGT1蛋白，研究其与血型系统(如P1PK系统)的关系，探索其在红细胞膜糖鞘脂合成中的作用，并开发基于该蛋白的血清学检测方法。
3. **文献名称**: *Structural Insights into GBGT1: Crystallization and Substrate Binding Mechanisms*
**作者**: Smith, T.J., et al.
**摘要**: 本研究解析了重组GBGT1的晶体结构，揭示了其催化结构域与底物结合的关键氨基酸残基，为设计特异性抑制剂提供了结构基础。
4. **文献名称**: *GBGT1 Overexpression in Cancer: Implications for Tumor Immune Evasion*
**作者**: Chen, L., et al.
**摘要**: 利用重组GBGT1蛋白模型，研究其在肿瘤细胞表面的异常表达如何通过糖基化修饰影响免疫识别，提出其作为潜在肿瘤标志物的可能性。
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**说明**：以上文献为示例性内容，实际引用时请通过 **PubMed**、**Google Scholar** 或 **Web of Science** 检索最新且准确的文献信息。
The recombinant human GBGT1 protein is a genetically engineered form of the globoside alpha-1.3-N-acetylgalactosaminyltransferase 1 enzyme, which plays a critical role in glycosphingolipid biosynthesis. GBGT1 catalyzes the transfer of N-acetylgalactosamine (GalNAc) to glycosphingolipid precursors, forming blood group system antigens such as P1 and NOR in the P1PK histo-blood group system. These antigens are expressed on red blood cells and other tissues, influencing cellular interactions, pathogen adhesion, and immune responses.
Due to its low natural abundance and tissue-specific expression, producing recombinant GBGT1 enables detailed functional and structural studies. It is typically expressed in mammalian cell systems (e.g., HEK293 or CHO cells) to ensure proper post-translational modifications. The purified protein is used to investigate enzyme kinetics, substrate specificity, and its role in diseases like paroxysmal nocturnal hemoglobinuria or autoimmune disorders linked to anti-P1 antibodies.
Recent research focuses on its potential involvement in cancer biology, microbial pathogenesis, and transfusion medicine. Structural elucidation of GBGT1 has provided insights into its catalytic mechanism and substrate recognition, aiding drug development targeting glycosylation disorders. Recombinant GBGT1 also serves as a critical reagent for diagnostic assays in blood typing and antibody detection.
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