| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GATAD1 |
| Uniprot No | Q8WUU5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-269aa |
| 氨基酸序列 | MPLGLKPTCSVCKTTSSSMWKKGAQGEILCHHCTGRGGAGSGGAGSGAAGGTGGSGGGGFGAATFASTSATPPQSNGGGGGKQSKQEIHRRSARLRNTKYKSAPAAEKKVSTKGKGRRHIFKLKNPIKAPESVSTIITAESIFYKGVYYQIGDVVSVIDEQDGKPYYAQIRGFIQDQYCEKSAALTWLIPTLSSPRDQFDPASYIIGPEEDLPRKMEYLEFVCHAPSEYFKSRSSPFPTVPTRPEKGYIWTHVGPTPAITIKESVANHL |
| 分子量 | 55.1 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为3篇关于重组人GATAD1蛋白的示例文献(部分为虚构参考，实际需通过学术数据库核实)：
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1. **文献名称**: "GATAD1 regulates DNA methylation through interaction with the NuRD complex"
**作者**: Smith J, et al.
**摘要**: 本研究利用重组人GATAD1蛋白揭示其通过结合NuRD复合体调控DNA甲基化修饰，影响染色质重塑和基因沉默的过程，为表观遗传学研究提供新机制。
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2. **文献名称**: "Structural analysis of human GATAD1 and its role in transcriptional repression"
**作者**: Li X, et al.
**摘要**: 通过重组表达GATAD1蛋白并进行晶体结构解析，发现其C端结构域对核定位和转录抑制功能至关重要，阐明其在癌细胞中异常表达的分子基础。
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3. **文献名称**: "Recombinant GATAD1 overexpression induces cell cycle arrest in non-small cell lung cancer"
**作者**: Wang Y, et al.
**摘要**: 构建重组人GATAD1真核表达载体并转染肺癌细胞，证明其过表达通过抑制cyclin D1通路导致G1期阻滞，提示其作为肿瘤抑制因子的潜在治疗价值。
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**注意**：以上文献为示例性内容，实际研究请通过PubMed、Google Scholar等平台搜索关键词“GATAD1 recombinant”或“GATAD1 protein function”获取真实参考文献。
GATAD1 (GATA Zinc Finger Domain Containing 1) is a conserved eukaryotic protein belonging to the GATA family-related protein subgroup. Unlike canonical GATA transcription factors that regulate gene expression via direct DNA binding, GATAD1 lacks a classic DNA-binding domain but retains a conserved GATA-type zinc finger motif. It is ubiquitously expressed, with elevated levels observed in tissues such as the heart, brain, and skeletal muscle. Functionally, GATAD1 interacts with chromatin-modifying complexes, including the NuRD (Nucleosome Remodeling and Deacetylase) complex, suggesting roles in chromatin remodeling and transcriptional repression. Studies implicate its involvement in cellular processes like myogenesis, neuronal development, and cardiac function. Dysregulation of GATAD1 has been linked to diseases such as dilated cardiomyopathy, intellectual disability, and cancer progression. Recombinant human GATAD1 protein is typically produced in Escherichia coli or mammalian expression systems, often fused with affinity tags (e.g., His-tag) for purification. This engineered protein serves as a critical tool for investigating molecular mechanisms, protein-protein interactions, and epigenetic regulation in disease models. Ongoing research aims to clarify its structural dynamics and therapeutic potential as a target for chromatin-related disorders.
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