| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GATS |
| Uniprot No | Q8NAP1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-163aa |
| 氨基酸序列 | MSCRGRGAGGRWNSTSWSTGCKLPASPRRVSRCSPTGLIKLAFLFSKTRCKFFSLTETPEDYTIIVDEEGFLELPSSEHLSVADATWLALNVVSGGGSFSSSQPIGMTKIAKSVIAPLADQNISVFMLSTYQTDFILVLKRDLPFVTHTLSSEFTILWSVARL |
| 分子量 | 44.2 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人人源GATS蛋白的参考文献示例(内容为虚构，仅用于格式演示)：
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1. **文献名称**：*"Structural and Functional Characterization of Recombinant Human GATS Protein in Transcriptional Regulation"*
**作者**：Zhang, L. et al. (2020)
**摘要**：本研究报道了重组人GATS蛋白的晶体结构解析，揭示了其通过C端锌指结构域与DNA特异性结合，调控下游基因转录的分子机制。体外实验表明，GATS通过招募组蛋白乙酰转移酶促进染色质重构。
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2. **文献名称**：*"GATS Protein as a Novel Biomarker in Triple-Negative Breast Cancer: Insights from Recombinant Protein-Based Assays"*
**作者**：Chen, H. et al. (2021)
**摘要**：通过重组表达的GATS蛋白开发了ELISA检测方法，发现其在三阴性乳腺癌患者血清中显著高表达。功能实验证实GATS通过激活Wnt/β-catenin通路促进肿瘤侵袭，提示其作为治疗靶点的潜力。
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3. **文献名称**：*"Recombinant Human GATS Protein Enhances Antiviral Immunity via STING Signaling Pathway"*
**作者**：Kim, J. & Patel, R. (2019)
**摘要**：该研究发现重组人GATS蛋白可与STING蛋白直接互作，促进Ⅰ型干扰素产生。通过小鼠模型证实，GATS蛋白处理显著抑制HSV-1病毒复制，为抗病毒免疫治疗提供新策略。
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4. **文献名称**：*"Large-Scale Production and Purification of Recombinant Human GATS Protein in HEK293 Cells"*
**作者**：Müller, F. et al. (2022)
**摘要**：优化了人源GATS蛋白在HEK293细胞中的重组表达工艺，建立三步层析纯化方案，获得高纯度(>95%)且具生物活性的蛋白，为后续功能研究和药物筛选提供可靠工具。
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(注：以上文献及内容均为模拟生成，实际研究中请参考真实学术数据库。)
**Background of Recombinant Human GATS Protein**
The GATA-binding protein (GATS), also known as GATA3. is a critical transcription factor involved in cellular differentiation, immune regulation, and development. It belongs to the GATA family, characterized by zinc finger domains that bind to DNA motifs with the consensus sequence (A/T)GATA(A/G). In humans, GATS plays pivotal roles in T-cell development, Th2 cell differentiation, and mammary gland morphogenesis. Dysregulation of GATS is linked to autoimmune diseases, allergies, and cancers, particularly breast and bladder carcinomas.
Recombinant human GATS protein is engineered via molecular cloning, typically expressed in *E. coli* or mammalian systems to ensure proper folding and post-translational modifications. This purified protein retains DNA-binding activity, making it invaluable for *in vitro* studies, including electrophoretic mobility shift assays (EMSAs), chromatin immunoprecipitation (ChIP), and functional investigations of gene regulation. Its production enables research into GATS-driven signaling pathways, therapeutic target validation, and diagnostic applications. By providing a consistent, high-purity product, recombinant GATS accelerates mechanistic studies and drug discovery efforts aimed at modulating its activity in disease contexts.
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