| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GATA6 |
| Uniprot No | Q92908 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-595aa |
| 氨基酸序列 | MALTDGGWCL PKRFGAAGAD ASDSRAFPAR EPSTPPSPIS SSSSSCSRGG ERGPGGASNC GTPQLDTEAA AGPPARSLLL SSYASHPFGA PHGPSAPGVA GPGGNLSSWE DLLLFTDLDQ AATASKLLWS SRGAKLSPFA PEQPEEMYQT LAALSSQGPA AYDGAPGGFV HSAAAAAAAA AAASSPVYVP TTRVGSMLPG LPYHLQGSGS GPANHAGGAG AHPGWPQASA DSPPYGSGGG AAGGGAAGPG GAGSAAAHVS ARFPYSPSPP MANGAAREPG GYAAAGSGGA GGVSGGGSSL AAMGGREPQY SSLSAARPLN GTYHHHHHHH HHHPSPYSPY VGAPLTPAWP AGPFETPVLH SLQSRAGAPL PVPRGPSADL LEDLSESREC VNCGSIQTPL WRRDGTGHYL CNACGLYSKM NGLSRPLIKP QKRVPSSRRL GLSCANCHTT TTTLWRRNAE GEPVCNACGL YMKLHGVPRP LAMKKEGIQT RKRKPKNINK SKTCSGNSNN SIPMTPTSTS SNSDDCSKNT SPTTQPTASG AGAPVMTGAG ESTNPENSEL KYSGQDGLYI GVSLASPAEV TSSVRPDSWC ALALA |
| 分子量 | 60.0 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人GATA6蛋白的示例参考文献(注：文献为虚拟构建，供参考)：
1. **"Recombinant Human GATA6 Protein Induces Pancreatic Progenitor Differentiation in vitro"**
**作者**: Zhang Y, Chen L, et al.
**摘要**: 研究利用大肠杆菌表达系统成功纯化重组人GATA6蛋白，并通过体外实验证实其能激活胰腺发育相关基因(如PDX1)，诱导多能干细胞向胰腺前体细胞分化。
2. **"Functional Characterization of GATA6 DNA-Binding Domains Using Recombinant Protein"**
**作者**: Kumar S, Kim H, et al.
**摘要**: 通过昆虫细胞系统表达带有GST标签的重组人GATA6蛋白，结合电泳迁移率实验(EMSA)和染色质免疫沉淀(ChIP)，解析其锌指结构域与靶基因启动子的相互作用机制。
3. **"GATA6 Suppresses Colorectal Cancer Metastasis via Interaction with β-Catenin"**
**作者**: Li X, Wang Q, et al.
**摘要**: 研究发现重组人GATA6蛋白通过抑制Wnt/β-catenin信号通路，降低上皮-间质转化(EMT)相关标志物表达，从而抑制结直肠癌细胞的侵袭能力。
4. **"High-Yield Purification of Recombinant Human GATA6 for Drug Screening Assays"**
**作者**: Patel R, Singh A, et al.
**摘要**: 开发了一种基于哺乳动物细胞的重组人GATA6蛋白规模化制备工艺，并应用于高通量药物筛选，发现小分子化合物可增强其转录调控活性。
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**注意**：以上文献为示例性内容，实际研究中建议通过PubMed、Google Scholar等平台检索关键词(如“recombinant human GATA6 protein”)获取真实文献。
Recombinant human GATA6 protein is a genetically engineered version of the transcription factor GATA6. which belongs to the GATA family of zinc finger proteins. GATA6 plays critical roles in embryonic development, particularly in the formation and differentiation of endodermal and mesodermal tissues, including the heart, pancreas, and gastrointestinal tract. It regulates gene expression by binding to GATA DNA motifs, influencing cellular processes like proliferation, apoptosis, and lineage specification. Dysregulation of GATA6 is linked to developmental disorders, cardiovascular defects, and cancers (e.g., pancreatic, colorectal).
Produced via bacterial, insect, or mammalian expression systems, recombinant GATA6 retains functional domains, including two zinc finger motifs essential for DNA interaction and protein partnerships. Its applications span mechanistic studies of organogenesis, disease modeling, and drug screening. Researchers use it to investigate GATA6’s role in stem cell differentiation, tissue regeneration, and signaling pathways like Wnt and BMP. Post-translational modifications (e.g., phosphorylation) may affect its activity, necessitating system-specific production for desired functionality. This tool bridges molecular insights with therapeutic potential in regenerative and precision medicine.
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