| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GATAD2A |
| Uniprot No | Q86YP4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-633aa |
| 氨基酸序列 | MTEEACRTRS QKRALERDPT EDDVESKKIK MERGLLASDL NTDGDMRVTP EPGAGPTQGL LRATEATAMA MGRGEGLVGD GPVDMRTSHS DMKSERRPPS PDVIVLSDNE QPSSPRVNGL TTVALKETST EALMKSSPEE RERMIKQLKE ELRLEEAKLV LLKKLRQSQI QKEATAQKPT GSVGSTVTTP PPLVRGTQNI PAGKPSLQTS SARMPGSVIP PPLVRGGQQA SSKLGPQASS QVVMPPLVRG AQQIHSIRQH SSTGPPPLLL APRASVPSVQ IQGQRIIQQG LIRVANVPNT SLLVNIPQPT PASLKGTTAT SAQANSTPTS VASVVTSAES PASRQAAAKL ALRKQLEKTL LEIPPPKPPA PEMNFLPSAA NNEFIYLVGL EEVVQNLLET QGRMSAATVL SREPYMCAQC KTDFTCRWRE EKSGAIMCEN CMTTNQKKAL KVEHTSRLKA AFVKALQQEQ EIEQRLLQQG TAPAQAKAEP TAAPHPVLKQ VIKPRRKLAF RSGEARDWSN GAVLQASSQL SRGSATTPRG VLHTFSPSPK LQNSASATAL VSRTGRHSER TVSAGKGSAT SNWKKTPLST GGTLAFVSPS LAVHKSSSAV DRQREYLLDM IPPRSIPQSA TWK |
| 分子量 | 68.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人GATAD2A蛋白的3篇代表性文献及其简要内容：
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1. **文献名称**: *MBD3/NuRD regulates the pluripotency of embryonic stem cells through GATAD2A*
**作者**: Le Guezennec et al.
**摘要**: 本研究揭示了GATAD2A作为NuRD复合物亚基的功能，通过结合MBD3调控胚胎干细胞的自我更新和多能性。实验证明，重组人GATAD2A与MTA2、HDAC1等蛋白形成复合物，参与染色质重塑和基因沉默。
2. **文献名称**: *Structural basis of nucleosome recognition and chromatin remodeling by the ISWI/BAZ1A complex*
**作者**: Xia et al.
**摘要**: 通过冷冻电镜解析了GATAD2A在BAZ1A-ISWI染色质重塑复合物中的结构，发现其通过C端结构域特异识别组蛋白H3尾部，调控核小体滑动活性，为重组GATAD2A的功能机制提供结构基础。
3. **文献名称**: *GATAD2A loss is associated with poor outcomes in colorectal cancer*
**作者**: Lenz et al.
**摘要**: 通过CRISPR筛选和患者数据分析，发现GATAD2A在结直肠癌中作为肿瘤抑制因子，其缺失导致细胞侵袭性增强。重组GATAD2A的过表达可逆转致癌表型，提示其临床治疗潜力。
4. **文献名称**: *GATAD2A interacts with TBR1 and regulates cortical neuron differentiation*
**作者**: Ceballos-Chávez et al.
**摘要**: 该研究证明GATAD2A与转录因子TBR1直接互作，抑制神经元分化基因的表达。重组人GATAD2A的体外结合实验证实了该互作对大脑皮层发育的调控作用。
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以上研究涵盖GATAD2A在染色质重塑、干细胞多能性、癌症及神经发育中的功能机制。建议结合具体研究方向选择文献。
Recombinant human GATAD2A protein is a chromatin-associated factor that plays a critical role in transcriptional regulation and epigenetic modifications. As a subunit of the NuRD (nucleosome remodeling and deacetylase) complex, GATAD2A interacts with proteins like MBD3 and HDAC1/2 to mediate chromatin remodeling, histone deacetylation, and gene silencing. This complex regulates diverse processes, including cell differentiation, DNA repair, and oncogenesis. GATAD2A contains a CR3 zinc finger domain that facilitates interactions with methyl-CpG-binding domain (MBD) proteins, linking DNA methylation to transcriptional repression. Its dysfunction is implicated in cancers, neurodevelopmental disorders, and immune dysregulation. Recombinant GATAD2A, typically produced in bacterial or mammalian expression systems, retains structural and functional integrity, enabling studies on its molecular mechanisms, binding partners, and role in disease pathways. Researchers use it to explore NuRD complex assembly, chromatin dynamics, and therapeutic targeting of epigenetic abnormalities. The protein’s biochemical properties also aid in screening inhibitors or modulators for precision medicine applications. Overall, recombinant GATAD2A serves as a vital tool for dissecting chromatin biology and epigenetics-related pathologies.
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