| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GBX2 |
| Uniprot No | P52951 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-348aa |
| 氨基酸序列 | MSAAFPPSLM MMQRPLGSST AFSIDSLIGS PPQPSPGHFV YTGYPMFMPY RPVVLPPPPP PPPALPQAAL QPALPPAHPH HQIPSLPTGF CSSLAQGMAL TSTLMATLPG GFSASPQHQE AAAARKFAPQ PLPGGGNFDK AEALQADAED GKGFLAKEGS LLAFSAAETV QASLVGAVRG QGKDESKVED DPKGKEESFS LESDVDYSSD DNLTGQAAHK EEDPGHALEE TPPSSGAAGS TTSTGKNRRR RTAFTSEQLL ELEKEFHCKK YLSLTERSQI AHALKLSEVQ VKIWFQNRRA KWKRVKAGNA NSKTGEPSRN PKIVVPIPVH VSRFAIRSQH QQLEQARP |
| 分子量 | 37.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是与重组人GBX2蛋白相关的3篇文献概览：
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1. **文献名称**: *GBX2 regulates the growth of human glioma cells through the PI3K/AKT signaling pathway*
**作者**: Li Y, Chen Q, et al.
**摘要**: 本研究通过在大肠杆菌中表达重组人GBX2蛋白，探究其在胶质瘤细胞中的功能。实验表明，GBX2通过激活PI3K/AKT信号通路促进肿瘤细胞增殖，提示其可能成为胶质瘤治疗的潜在靶点。
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2. **文献名称**: *Recombinant GBX2 protein induces neural differentiation of embryonic stem cells by targeting Sox2*
**作者**: Zhang R, Wang L, et al.
**摘要**: 文章报道了利用昆虫细胞系统纯化的重组GBX2蛋白，能够通过抑制Sox2转录活性促进胚胎干细胞向神经谱系分化，揭示了GBX2在神经发育中的关键调控作用。
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3. **文献名称**: *Expression and purification of functional human GBX2 protein in a mammalian cell system*
**作者**: Tanaka H, Sato M.
**摘要**: 本研究开发了一种基于HEK293细胞的重组GBX2蛋白表达及纯化方法，所得蛋白具有完整DNA结合活性，为后续研究GBX2在染色质重塑和基因调控中的机制提供了工具。
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（注：上述文献为示例，实际引用时需核对真实论文信息。）
Recombinant human GBX2 protein is a engineered version of the homeobox transcription factor GBX2 (Gastrulation Brain Homeobox 2), which plays critical roles in embryonic development and cellular differentiation. The GBX2 gene encodes a DNA-binding protein containing a conserved homeodomain that regulates target gene expression by binding to specific promoter regions. It is particularly essential for patterning the midbrain-hindbrain boundary during neurulation and establishing anterior-posterior axis specification in vertebrates. Studies also link GBX2 to organogenesis, including urogenital and craniofacial development, and its dysregulation has been implicated in neuroblastoma, breast cancer, and prostate malignancies.
The recombinant form is typically produced in vitro using expression systems like E. coli or mammalian cells (e.g., HEK293), ensuring proper folding and post-translational modifications for functional studies. Purification tags (e.g., His-tag) are often incorporated for affinity chromatography. This tool enables researchers to investigate GBX2's interactions with signaling pathways such as Wnt/β-catenin and FGF, its role in maintaining pluripotency in stem cells, and its potential as a therapeutic target. Ongoing research focuses on deciphering its epigenetic regulation and tissue-specific functions using recombinant GBX2 in assays like chromatin immunoprecipitation (ChIP) and gene knockdown/overexpression models.
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