| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GBX1 |
| Uniprot No | Q14549 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-363aa |
| 氨基酸序列 | MQRAGGGSAPGGNGGGGGGGPGTAFSIDSLIGPPPPRSGHLLYTGYPMFMPYRPLVLPQALAPAPLPAGLPPLAPLASFAGRLTNTFCAGLGQAVPSMVALTTALPSFAEPPDAFYGPQELAAAAAAAAATAARNNPEPGGRRPEGGLEADELLPAREKVAEPPPPPPPHFSETFPSLPAEGKVYSSDEEKLEASAGDPAGSEQEEEGSGGDSEDDGFLDSSAGGPGALLGPKPKLKGSLGTGAEEGAPVTAGVTAPGGKSRRRRTAFTSEQLLELEKEFHCKKYLSLTERSQIAHALKLSEVQVKIWFQNRRAKWKRIKAGNVSSRSGEPVRNPKIVVPIPVHVNRFAVRSQHQQMEQGARP |
| 分子量 | 66.33 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人GBX1蛋白的3篇参考文献摘要(文献信息为模拟示例，实际文献需根据数据库查询)：
1. **文献名称**: *Functional analysis of recombinant human GBX1 protein in neural cell differentiation*
**作者**: Zhang L, et al.
**摘要**: 本研究利用大肠杆菌系统表达重组人GBX1蛋白，验证其可促进小鼠神经干细胞向神经元分化的功能，揭示其在神经发育中的潜在作用。
2. **文献名称**: *Structural characterization and DNA-binding properties of GBX1 homeodomain protein*
**作者**: Kimura T, et al.
**摘要**: 通过哺乳动物细胞表达纯化重组人GBX1蛋白，解析其与靶DNA序列的结合特性，证明其C端结构域对调控基因转录具有关键影响。
3. **文献名称**: *GBX1 knockdown and overexpression models implicate its role in tumor metastasis suppression*
**作者**: Wang Y, et al.
**摘要**: 研究利用重组GBX1蛋白处理乳腺癌细胞，发现其通过调控EMT通路显著抑制癌细胞迁移，提示其在肿瘤治疗中的潜在应用价值。
注：以上内容为示例，实际文献需通过PubMed、Google Scholar等平台以关键词“recombinant GBX1”或“GBX1 protein function”查询真实研究。
Recombinant human GBX1 protein is a genetically engineered form of the GBX1 (Gastrulation Brain Homeobox 1) protein, which belongs to the homeobox gene family. GBX1 encodes a transcription factor containing a highly conserved homeodomain, enabling sequence-specific DNA binding to regulate target gene expression. It plays roles in embryonic development, particularly in neural patterning, midbrain-hindbrain boundary formation, and cell differentiation. Studies suggest its involvement in signaling pathways like Wnt and Shh, though its precise biological functions remain under investigation.
The recombinant protein is typically produced using expression systems such as E. coli or mammalian cells, ensuring post-translational modifications critical for activity. Researchers utilize it to explore GBX1's molecular mechanisms, protein-DNA interactions, and its potential role in diseases, including neurological disorders or cancers. Current challenges include clarifying its context-dependent regulatory networks and tissue-specific functions. Recombinant GBX1 serves as a vital tool for structural studies, antibody development, and functional assays, bridging gaps in understanding developmental biology and disease pathogenesis.
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