| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GLB1L |
| Uniprot No | Q6UWU2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-654aa |
| 氨基酸序列 | MAPKKLSCLRSLLLPLSLTLLLPQADTRSFVVDRGHDRFLLDGAPFRYVSGSLHYFRVPRVLWADRLLKMRWSGLNAIQFYVPWNYHEPQPGVYNFNGSRDLIAFLNEAALANLLVILRPGPYICAEWEMGGLPSWLLRKPEIHLRTSDPDFLAAVDSWFKVLLPKIYPWLYHNGGNIISIQVENEYGSYRACDFSYMRHLAGLFRALLGEKILLFTTDGPEGLKCGSLRGLYTTVDFGPADNMTKIFTLLRKYEPHGPLVNSEYYTGWLDYWGQNHSTRSVSAVTKGLENMLKLGASVNMYMFHGGTNFGYWNGADKKGRFLPITTSYDYDAPISEAGDPTPKLFALRDVISKFQEVPLGPLPPPSPKMMLGPVTLHLVGHLLAFLDLLCPRGPIHSILPMTFEAVKQDHGFMLYRTYMTHTIFEPTPFWVPNNGVHDRAYVMVDGVFQGVVERNMRDKLFLTGKLGSKLDILVENMGRLSFGSNSSDFKGLLKPPILGQTILTQWMMFPLKIDNLVKWWFPLQLPKWPYPQAPSGPTFYSKTFPILGSVGDTFLYLPGWTKGQVWINGFNLGRYWTKQGPQQTLYVPRFLLFPRGALNKITLLELEDVPLQPQVQFLDKPILNSTSTLHRTHINSLSADTLSASEPMELSGH |
| 分子量 | 100.6 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人GLB1L蛋白的3篇参考文献（均为虚构示例，仅供参考格式）：
1. **文献名称**: *Expression and Characterization of Recombinant Human GLB1L Protein in Escherichia coli*
**作者**: Zhang Y, et al.
**摘要**: 研究报道了在大肠杆菌中成功表达并纯化重组人GLB1L蛋白，通过亲和层析获得高纯度蛋白，并验证其具有β-半乳糖苷酶活性，为进一步功能研究奠定基础。
2. **文献名称**: *Structural Insights into Human GLB1L Protein by Cryo-EM Analysis*
**作者**: Wang X, et al.
**摘要**: 利用冷冻电镜技术解析了重组人GLB1L蛋白的三维结构，揭示了其催化活性位点的关键氨基酸残基，并提出其与溶酶体底物特异性结合的分子机制。
3. **文献名称**: *Functional Role of GLB1L in Lysosomal Disorders: In vitro Studies Using Recombinant Protein*
**作者**: Liu J, et al.
**摘要**: 通过体外实验证明重组人GLB1L蛋白可修复模型细胞中β-半乳糖苷酶活性缺陷，提示其在GM1神经节苷脂贮积症等溶酶体疾病中的潜在治疗作用。
*注：以上文献为模拟内容，实际研究中请根据具体论文调整。建议通过PubMed或Google Scholar检索真实文献。*
Recombinant human GLB1L protein is a genetically engineered form of β-galactosidase-1-like (GLB1L), a member of the glycoside hydrolase family. GLB1L shares structural homology with β-galactosidase (GLB1), an enzyme critical for lysosomal degradation of glycoproteins and glycolipids, but its precise biological role remains less characterized. Unlike GLB1, mutations in which cause GM1-gangliosidosis, GLB1L is not directly linked to known lysosomal storage disorders. It may function in glycoconjugate processing or cellular signaling, though further studies are needed.
Produced via recombinant DNA technology, the protein is typically expressed in mammalian or insect cell systems to ensure proper post-translational modifications and folding. The coding sequence is cloned into expression vectors, followed by transfection, purification (e.g., affinity tags), and validation via SDS-PAGE or enzymatic assays. Recombinant GLB1L enables biochemical studies, including substrate specificity profiling, structural analysis, and interaction mapping. Its role in diseases like cancer or neurodegenerative conditions is under exploration, given potential links to altered glycosylation pathways. Research on recombinant GLB1L aids in deciphering its physiological function and therapeutic potential.
×
